Dimethyl sulfoxide inactivates the anticancer effect of cisplatin against human myelogenous leukemia cell lines in in vitro assays

• Published in: Indian journal of pharmacology Vol. 47; no. 3; pp. 322 - 324
• Main Authors: Raghavan, Rahul, Cheriyamundath, Sanith, Madassery, Joseph
• Format: Journal Article
• Language: English
• Published: India Medknow Publications and Media Pvt. Ltd 01.05.2015; Medknow Publications & Media Pvt. Ltd; Medknow Publications & Media Pvt Ltd
• Subjects: Bioassay; Cancer; Cancer treatment; Cell Line, Tumor; Cell lines; Cell Survival - drug effects; Cellular biology; Cisplatin; Cisplatin - antagonists & inhibitors; Cisplatin - chemistry; Cisplatin - pharmacology; Deoxyribonucleic acid; Dimethyl sulfoxide; Dimethyl Sulfoxide - chemistry; Dimethyl Sulfoxide - pharmacology; DNA; Dosage and administration; Dose-Response Relationship, Drug; Drug Interactions; Drug therapy; Health aspects; Humans; Leukemia; Leukemia, Myeloid - pathology; Methods; Short Communication; DMSO; Cisplatin; K562 cell line
• ISSN: 0253-7613; 1998-3751
• DOI: 10.4103/0253-7613.157132

To investigate the effect of DMSO on cisplatin induced cytotoxicity (invitro) against K562 (Human mylogenous leukemia) cell line and to study the cisplatin-DMSO adduct formation using UV-spectrophotometer. Effect of DMSO on the cytotoxicity of cisplatin was studied in K562 (Chronic mylogenous leukemia) cell line by MTT assay. Cisplatin-DMSO adduct formation was studied by continuously monitoring the increase in absorption peaks for 30 minutes using UV-spectrophotometer. 0.1-0.3% DMSO markedly reduced the cytotoxic activity of cisplatin in K562 cells. Cisplatin-DMSO adduct formation was detected using UV-spectrophotometer. Continuous increase in UV absorbance between 250nm-290nm was observed when cisplatin (0.5mg/ml) and DMSO (10%) were mixed. Present study revealed that DMSO inactivates the cytotoxicity of cisplatin. Cisplatin-DMSO mixture showed increased absorbance at 250-290nm. Therefore, using DMSO in invitro assays might result in misinterpretation of actual efficacy of drugs.