Impact of global PTP1B deficiency on the gut barrier permeability during NASH in mice

• Published in: Molecular metabolism (Germany) Vol. 35; p. 100954
• Main Authors: Rubio, Carmen, Puerto, Marta, García-Rodríquez, Juan J., Lu, Van B., García-Martínez, Irma, Alén, Rosa, Sanmartín-Salinas, Patricia, Toledo-Lobo, M. Val, Saiz, Jorge, Ruperez, Javier, Barbas, Coral, Menchén, Luis, Gribble, Fiona M., Reimann, Frank, Guijarro, Luis G., Carrascosa, Jose M., Valverde, Ángela M.
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.05.2020; Elsevier
• Subjects: Animals; Choline Deficiency - complications; Diet - adverse effects; Disease Models, Animal; Gastrointestinal Microbiome - genetics; Gene Expression; Gene Knockout Techniques; GLP-1; GLP-2; Glucagon-Like Peptide 1 - blood; Gut microbiota; Inflammation; Inflammation - metabolism; Intestinal Mucosa - metabolism; Liver - metabolism; Male; Methionine - deficiency; Mice; Mice, Inbred C57BL; Mice, Knockout; NASH; Non-alcoholic Fatty Liver Disease - etiology; Non-alcoholic Fatty Liver Disease - metabolism; Original; Permeability; Protein Tyrosine Phosphatase, Non-Receptor Type 1 - deficiency; Protein Tyrosine Phosphatase, Non-Receptor Type 1 - genetics; PTP1B; RAW 264.7 Cells; Gut microbiota; Inflammation; PTP1B; GLP-1; NASH; GLP-2
• ISSN: 2212-8778; 2212-8778
• DOI: 10.1016/j.molmet.2020.01.018

Non-alcoholic steatohepatitis (NASH) is characterized by a robust pro-inflammatory component at both hepatic and systemic levels together with a disease-specific gut microbiome signature. Protein tyrosine phosphatase 1 B (PTP1B) plays distinct roles in non-immune and immune cells, in the latter inhibiting pro-inflammatory signaling cascades. In this study, we have explored the role of PTP1B in the composition of gut microbiota and gut barrier dynamics in methionine and choline-deficient (MCD) diet-induced NASH in mice. Gut features and barrier permeability were characterized in wild-type (PTP1B WT) and PTP1B-deficient knockout (PTP1B KO) mice fed a chow or methionine/choline-deficient (MCD) diet for 4 weeks. The impact of inflammation was studied in intestinal epithelial and enteroendocrine cells. The secretion of GLP-1 was evaluated in primary colonic cultures and plasma of mice. We found that a shift in the gut microbiota shape, disruption of gut barrier function, higher levels of serum bile acids, and decreased circulating glucagon-like peptide (GLP)-1 are features during NASH. Surprisingly, despite the pro-inflammatory phenotype of global PTP1B-deficient mice, they were partly protected against the alterations in gut microbiota composition during NASH and presented better gut barrier integrity and less permeability under this pathological condition. These effects concurred with higher colonic mucosal inflammation, decreased serum bile acids, and protection against the decrease in circulating GLP-1 levels during NASH compared with their WT counterparts together with increased expression of GLP-2-sensitive genes in the gut. At the molecular level, stimulation of enteroendocrine STC-1 cells with a pro-inflammatory conditioned medium (CM) from lipopolysaccharide (LPS)-stimulated macrophages triggered pro-inflammatory signaling cascades that were further exacerbated by a PTP1B inhibitor. Likewise, the pro-inflammatory CM induced GLP-1 secretion in primary colonic cultures, an effect augmented by PTP1B inhibition. Altogether our results have unraveled a potential role of PTP1B in the gut–liver axis during NASH, likely mediated by increased sensitivity to GLPs, with potential therapeutic value. [Display omitted] •MCD diet induces a different shift of gut microbiota in PTP1BWT and PTP1B KO mice.•PTP1B KO mice present higher colonic mucosal inflammation under NASH conditions.•Gut barrier permeability was decreased in PTP1B KO mice with NASH.•PTP1B inhibition increases GLP-1 secretion in colonic cultures exposed to inflammatory stimulus.