Simultaneous extraction and separation of compounds from mate (Ilex paraguariensis) leaves by pressurized liquid extraction coupled with solid-phase extraction and in-line UV detection

• Published in: Food chemistry. Molecular sciences Vol. 2; p. 100008
• Main Authors: Souza, Mariana C., Silva, Laise C., Chaves, Jaisa O., Salvador, Mayara P., Sanches, Vitor L., da Cunha, Diogo T., Foster Carneiro, Tânia, Rostagno, Mauricio A.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 30.07.2021; Elsevier
• Subjects: Caffeine; Flavonoids; from the Special Issue ‘Food bioactives in human health and nutrition’ by Matilde D’Arrigo, Daniel Cozzolino and Yasmina Sultanbawa; Mate; On-line; Phenolic acids; Pressurized-liquid extraction; Solid-phase extraction; UV detection; Mate; Pressurized-liquid extraction; Flavonoids; UV detection; On-line; Solid-phase extraction; Phenolic acids; Caffeine
• ISSN: 2666-5662; 2666-5662
• DOI: 10.1016/j.fochms.2020.100008

•pH and temperature are the main variables affecting recovery and separation.•The selection of the adsorbent is critical for the recovery of less polar compounds.•Excellent separation of compounds in different fractions was achieved.•The use of a UV–Vis detector allowed monitoring the process in real-time.•The developed method provided higher recoveries than conventional methods. The in-line coupling of the pressurized liquid extraction with a solid-phase adsorbent and a UV–Vis detector for the simultaneous extraction and separation of bioactive compounds from yerba mate (PLE-SPE-UV) was carried out in two stages. In the first stage, water was used as a solvent, while in the second stage, ethanol was used. For the optimization of the method, different adsorbents (Sepra C18-E, Isolute C18-EC, and Strata-X C18), temperatures (40–80 °C), solvent flow-rate (1–3 mL/min), and pH (4.0 and 8.0) were evaluated. By using a UV–Vis detector on-line, it is possible to monitor the process in real-time. The developed method allowed obtaining similar or higher recoveries of all the compounds classes than other methods, such as ultrasound-assisted extraction, stirring, maceration, and pressurized liquid extraction alone, in addition to separating them into fractions. The developed method could be used as sample preparation for the analysis of different compounds classes from mate.