Regulation of UCP1 and Mitochondrial Metabolism in Brown Adipose Tissue by Reversible Succinylation

• Published in: Molecular cell Vol. 74; no. 4; pp. 844 - 857.e7
• Main Authors: Wang, GuoXiao, Meyer, Jesse G., Cai, Weikang, Softic, Samir, Li, Mengyao Ella, Verdin, Eric, Newgard, Christopher, Schilling, Birgit, Kahn, C. Ronald
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 16.05.2019
• Subjects: Adipose Tissue, Brown - metabolism; Adipose Tissue, Brown - pathology; Animals; brown fat; Energy Metabolism - genetics; Gene Expression Regulation; Glucose - metabolism; Mice; Mice, Knockout; mitochondria; Mitochondria - genetics; Mitochondria - metabolism; Mitochondrial Proteins - genetics; Obesity - genetics; Obesity - metabolism; Obesity - pathology; Proteomics - methods; Sirtuins - genetics; Succinic Acid - metabolism; succinylation; thermogenesis; Thermogenesis - genetics; UCP1; Uncoupling Protein 1 - genetics; Uncoupling Protein 1 - metabolism; UCP1; thermogenesis; succinylation; brown fat; mitochondria
• ISSN: 1097-2765; 1097-4164
• DOI: 10.1016/j.molcel.2019.03.021

Brown adipose tissue (BAT) is rich in mitochondria and plays important roles in energy expenditure, thermogenesis, and glucose homeostasis. We find that levels of mitochondrial protein succinylation and malonylation are high in BAT and subject to physiological and genetic regulation. BAT-specific deletion of Sirt5, a mitochondrial desuccinylase and demalonylase, results in dramatic increases in global protein succinylation and malonylation. Mass spectrometry-based quantification of succinylation reveals that Sirt5 regulates the key thermogenic protein in BAT, UCP1. Mutation of the two succinylated lysines in UCP1 to acyl-mimetic glutamine and glutamic acid significantly decreases its stability and activity. The reduced function of UCP1 and other proteins in Sirt5KO BAT results in impaired mitochondria respiration, defective mitophagy, and metabolic inflexibility. Thus, succinylation of UCP1 and other mitochondrial proteins plays an important role in BAT and in regulation of energy homeostasis. [Display omitted] •Sirt5 regulates mitochondrial protein succinylation and malonylation in brown fat•Increased succinylation of UCP1 reduces its stability and function•Sirt5KO in BAT leads to metabolic inflexibility and impairs mitochondrial homeostasis•These processes are altered by cold exposure and diet Wang et al. performed succinyl-proteomics in brown fat (BAT) of normal and Sirt5 KO mice and identified UCP1 as a new target of Sirt5 desuccinylation. UCP1 with succinyl-mimetic mutations displayed reduced activity and stability. Elevated succinylation of mitochondrial protein in Sirt5 KO BAT resulted in altered metabolic flexibility and mitophagy.