Application of fluorescence spectroscopy using a novel fluoroionophore for quantification of zinc in urban runoff

• Published in: Water research (Oxford) Vol. 54; pp. 12 - 20
• Main Authors: Hafuka, Akira, Yoshikawa, Hiroaki, Yamada, Koji, Kato, Tsuyoshi, Takahashi, Masahiro, Okabe, Satoshi, Satoh, Hisashi
• Format: Journal Article
• Language: English
• Published: England 01.05.2014
• Subjects: Absorption; Carbon; Carbon - chemistry; Cities; Dissolution; Environmental extremism; Fluorescence; Hydrogen-Ion Concentration; Ionophores - chemistry; Ions; Japan; Metals - chemistry; Spectrometry, Fluorescence - methods; Spectroscopy; Urban runoff; Water Pollutants, Chemical - analysis; Zinc; Zinc - analysis; Japan; Fluorescence spectroscopy; Fluoroionophore; Spearman's rank correlation analysis; Urban runoff; Zinc
• ISSN: 0043-1354; 1879-2448
• DOI: 10.1016/j.watres.2014.01.040

Fluorescence spectroscopy has great potential for on-site and real-time monitoring of pollutants in aquatic environments; however, its application to environmental aquatic samples has been extremely limited. In this study, a novel fluoroionophore based on a BODIPY-terpyridine conjugate was developed and applied to determine Zn concentrations in urban runoff. The fluoroionophore selectively bound to Zn(2+) in water, which led to an instant red-shift of the fluorescence peak of the fluoroionophore from 539 nm to 567 nm that could be seen by the naked eye. Zn concentrations could be quantified using the ratio of fluorescence intensities, and the detection limit was 9 μg/L, which is sufficiently low for environmental aquatic samples. To demonstrate applicability of the method to environmental samples, we measured Zn concentrations in urban runoff samples with a complex matrix (∼60 mg/L dissolved organic carbon and ∼20 mS/cm electrical conductivity). The total and dissolved fractions of Zn in the samples could be determined by fluorescence spectroscopy and its relative error was estimated to be less than 30% by inductively coupled plasma-atomic emission spectroscopy analysis. The proposed method is rapid and easy-to-use with simple pretreatment for Zn determination in environmental aquatic samples with complex matrices.