Co-occurrence of colistin-resistance genes mcr-1 and mcr-3 among multidrug-resistant Escherichia coli isolated from cattle, Spain, September 2015

• Published in: Euro surveillance : bulletin européen sur les maladies transmissibles Vol. 22; no. 31; p. 13
• Main Authors: Hernández, Marta, Iglesias, M Rocío, Rodríguez-Lázaro, David, Gallardo, Alejandro, Quijada, Narciso, Miguela-Villoldo, Pedro, Campos, Maria Jorge, Píriz, Segundo, López-Orozco, Gema, de Frutos, Cristina, Sáez, José Luis, Ugarte-Ruiz, María, Domínguez, Lucas, Quesada, Alberto
• Format: Journal Article
• Language: English
• Published: Sweden Centre Europeen pour la Surveillance Epidemiologique du SIDA (European Centre for the Epidemiological Monitoring of AIDS) 03.08.2017; European Centre for Disease Prevention and Control (ECDC)
• Subjects: Animals; Anti-Bacterial Agents - administration & dosage; Anti-Bacterial Agents - pharmacology; Antibiotics; beta-Lactamases - genetics; Cattle; Cattle - microbiology; Colistin - administration & dosage; Colistin - pharmacology; Drug resistance; Drug Resistance, Bacterial - genetics; E coli; Escherichia coli - drug effects; Escherichia coli - genetics; Escherichia coli - isolation & purification; Escherichia coli Infections - microbiology; Escherichia coli Infections - veterinary; Escherichia coli Proteins - genetics; Feces - microbiology; Genes; Microbial Sensitivity Tests; Molecular Typing; Multidrug resistant organisms; Peptides; Polymerase Chain Reaction; Rapid Communication; Sentinel Surveillance; Spain; antimicrobial resistance; Escherichia coli; bovine; cattle; mcr-3; colistin; mcr-1
• ISSN: 1560-7917; 1025-496X; 1560-7917
• DOI: 10.2807/1560-7917.es.2017.22.31.30586

Colistin resistance genes mcr-3 and mcr-1 have been detected in an Escherichia coli isolate from cattle faeces in a Spanish slaughterhouse in 2015. The sequences of both genes hybridised to same plasmid band of ca 250 kb, although colistin resistance was non-mobilisable. The isolate was producing extended-spectrum beta-lactamases and belonged to serotype O9:H10 and sequence type ST533. Here we report an mcr-3 gene detected in Europe following earlier reports from Asia and the United States.