Hepatoprotective effect of food preservatives (butylated hydroxyanisole, butylated hydroxytoluene) on carbon tetrachloride-induced hepatotoxicity in rat

• Published in: Toxicology reports Vol. 5; pp. 31 - 37
• Main Authors: Dassarma, Barsha, Nandi, Dilip K., Gangopadhyay, Somnath, Samanta, Saptadip
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 01.01.2018; Elsevier
• Subjects: Butylated hydroxyanisole; Butylated hydroxytoluene; CCl4 - intoxicated rats; Hepatoprotection; Hepatoprotection; Butylated hydroxyanisole; CCl4 - intoxicated rats; Butylated hydroxytoluene
• ISSN: 2214-7500; 2214-7500
• DOI: 10.1016/j.toxrep.2017.12.009

[Display omitted] •The report about dose (230mg/kg body wt/rat/day) that had been used for development of hepatotoxicity in male albino rat (Wister strain) was taken from our own previous study and it had never been used by any other group of researchers.•This study had shown the detailed results of ROS mediated hepatotoxicity and oxidative stress profiles which was one of the important reports among the previous findings.•GARS grade synthetic food preservative BHA, BHT could be able to reduce the hepatotoxicity in male albino (Wister strain) rats and it is the first time report in our study. Carbon tetrachloride (CCl4), a hepatotoxic agent is widely used to study the toxic mechanisms in experimental animals. This study was carried out to establish the hepatoprotective measures of food preservative antioxidants butylated hydroxyanisole and butylated hydroxytolune (BHA, BHT) when mixed with food towards carbon tetrachloride (CCl4) intoxication (230mg/kg b wt/rat/day) in rat. Biochemical markers like serum glutamate pyruvate tranaminase (AST), serum glutamate oxaloacetate transaminase (ALT), alkaline phosphatase (ALP) and bilirubin content, antioxidant enzymes such as SOD, CAT, GPx, and malondialdehyde (MDA) as the end product of lipid peroxidanion were measured. The results had shown the elevated level of AST (121.16%), ALT (124.68%), ALP (122.41%) an, bilirubin content (57.14%) after CCl4 treatment. Marked decrease of activity of antioxidant enzymes such as SOD (85.36%), CAT (67.47%), GPx (50.7%) had indicated that the ROS mediated toxicity and pretreatment of BHA and BHT restored the activity of these enzymes. High level of MDA content with reduced GSH value was also observed due to oxidative stress. The hepatic antioxidant status was restored with the food preservative (BHA, BHT) antioxidant treatment which had indicated the significant protective effect against CCl4 induced hepatotoxicity and finally confirmed by histopathological studies.