Spatial-Temporal Lineage Restrictions of Embryonic p63+ Progenitors Establish Distinct Stem Cell Pools in Adult Airways

• Published in: Developmental cell Vol. 44; no. 6; pp. 752 - 761.e4
• Main Authors: Yang, Ying, Riccio, Paul, Schotsaert, Michael, Mori, Munemasa, Lu, Jining, Lee, Dong-Kee, García-Sastre, Adolfo, Xu, Jianming, Cardoso, Wellington V.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 26.03.2018
• Subjects: airway progenitors; Animals; basal cells; Cell Differentiation; Cell Lineage; Cells, Cultured; Embryonic Stem Cells - cytology; Embryonic Stem Cells - metabolism; Embryonic Stem Cells - virology; Female; H1N1; influenza A; Influenza A Virus, H1N1 Subtype - physiology; lineage tracing; Lung - cytology; Lung - metabolism; Lung - virology; Male; Mice; Mice, Knockout; p63; Phosphoproteins - physiology; Respiratory Mucosa - cytology; Respiratory Mucosa - metabolism; Respiratory Mucosa - virology; stem cell; Stem Cells - cytology; Stem Cells - metabolism; Stem Cells - virology; Trachea - cytology; Trachea - metabolism; Trachea - virology; Trans-Activators - physiology; influenza A; stem cell; airway progenitors; lineage tracing; p63; H1N1; basal cells
• ISSN: 1534-5807; 1878-1551
• DOI: 10.1016/j.devcel.2018.03.001

Basal cells (BCs) are p63-expressing multipotent progenitors of skin, tracheoesophageal and urinary tracts. p63 is abundant in developing airways; however, it remains largely unclear how embryonic p63+ cells contribute to the developing and postnatal respiratory tract epithelium, and ultimately how they relate to adult BCs. Using lineage-tracing and functional approaches in vivo, we show that p63+ cells arising from the lung primordium are initially multipotent progenitors of airway and alveolar lineages but later become restricted proximally to generate the tracheal adult stem cell pool. In intrapulmonary airways, these cells are maintained immature to adulthood in bronchi, establishing a rare p63+Krt5− progenitor cell population that responds to H1N1 virus-induced severe injury. Intriguingly, this pool includes a CC10 lineage-labeled p63+Krt5− cell subpopulation required for a full H1N1-response. These data elucidate key aspects in the establishment of regionally distinct adult stem cell pools in the respiratory system, potentially with relevance to other organs. [Display omitted] •Embryonic p63+ cells before E10.5 generate both airway and alveolar descendants•Further p63 lineage restriction at E13.5 defines the tracheal basal progenitor pool•H1N1-induced Krt5+ pods originate from the embryonic intrapulmonary p63+ progenitors•Intrapulmonary CC10 lineage-labeled p63+ cells are required for a full H1N1 response Yang et al. show that embryonic p63+ cells are initially multipotent progenitors of airways and alveoli. Later, however, they become proximally restricted to generate tracheal basal cells and an intrapulmonary p63+Krt5− progenitor pool that is maintained immature to adulthood. This pool contains p63+CC10Lineage+ cells and mediates H1N1 virus-induced pathological remodeling.