Oligodendrocytes Support Neuronal Glutamatergic Transmission via Expression of Glutamine Synthetase

• Published in: Cell reports (Cambridge) Vol. 27; no. 8; pp. 2262 - 2271.e5
• Main Authors: Xin, Wendy, Mironova, Yevgeniya A., Shen, Hui, Marino, Rosa A.M., Waisman, Ari, Lamers, Wouter H., Bergles, Dwight E., Bonci, Antonello
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 21.05.2019; Elsevier
• Subjects: Animals; Brain - physiopathology; cocaine; glia; glutamate; Glutamate-Ammonia Ligase - metabolism; glutamine; Glutamine - metabolism; glutamine synthetase; midbrain; oligodendrocyte; Oligodendroglia - metabolism; Signal Transduction; transmission; transmission; midbrain; cocaine; glutamine synthetase; oligodendrocyte; glutamine; glia; glutamate
• ISSN: 2211-1247; 2211-1247
• DOI: 10.1016/j.celrep.2019.04.094

Glutamate has been implicated in a wide range of brain pathologies and is thought to be metabolized via the astrocyte-specific enzyme glutamine synthetase (GS). We show here that oligodendrocytes, the myelinating glia of the central nervous system, also express high levels of GS in caudal regions like the midbrain and the spinal cord. Selective removal of oligodendrocyte GS in mice led to reduced brain glutamate and glutamine levels and impaired glutamatergic synaptic transmission without disrupting myelination. Furthermore, animals lacking oligodendrocyte GS displayed deficits in cocaine-induced locomotor sensitization, a behavior that is dependent on glutamatergic signaling in the midbrain. Thus, oligodendrocytes support glutamatergic transmission through the actions of GS and may represent a therapeutic target for pathological conditions related to brain glutamate dysregulation. [Display omitted] •Oligodendrocytes (OLs) express glutamine synthetase (GS)•OL GS expression is low in rostral regions and high in caudal regions of the brain•OLs do not require GS for survival or myelination•Loss of OL GS disrupts neuronal glutamate signaling and glutamate-dependent behavior Xin et al. show that mature oligodendrocytes, the myelinating cells of the brain, express the glutamine-synthesizing enzyme glutamine synthetase (GS). Oligodendrocyte-specific GS deletion does not impair myelination but disrupts neuronal glutamatergic transmission, thus demonstrating a myelin-independent role for oligodendrocytes in supporting glutamate signaling in the brain.