Myorelaxation, respiratory depression and electrocardiographic changes caused by the administration of extract of açai (Euterpe oleracea Mart.) stone in rats

• Published in: Toxicology reports Vol. 8; pp. 829 - 838
• Main Authors: Muto, Nilton Akio, Hamoy, Moisés, Rodrigues Lucas, David Cristian, Teixeira, Bruno Brito, Santos Almeida, Adrielle Felicia, de Castro Navegantes, Thamires, de Sousa Ferreira de Sá, Vaniza Sheila, de Moraes, Brenda Pinto, do Vale Medeiros, João Paulo, dos Santos, Yasmin Amorim, da Rocha, Claúdia Quintino, de Mello, Vanessa Joia, Rogez, Hervé
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 01.01.2021; Elsevier
• Subjects: ECG; EMG; Euterpe oleracea; Myorelaxant; Regular; Sedative; ACB; mg GAE/g DE; EEOS; ESI-IT-MS; ECG; CNS; Euterpe oleracea; mg CAE/g DE; GABAA; RC; RD; DMACA; EMGs; RPR; mg MRE/g DE; Myorelaxant; mg CE/g DE; DZP; Sedative; EMG; HPLC; RD, respiratory depression; mg CE/g DE, milligrams of cyanidin equivalents per gram of dried extract; ACB, abdominal-costal breathing; mg GAE/g DE, milligrams of gallic acid equivalents per gram of dried extract; EMG, electromyographic; HPLC, High Performance Liquid Chromatography; CNS, Central Nervous System; mg CAE/g DE, milligrams of catechin equivalents per gram of dried extract; DZP, diazepam; EEOS, extract of E. oleracea stone; mg MRE/g DE, miligrams of myricetin-3-O-α-l-rhamnopyranoside equivalents per gram of dried extract; EMGs, electromyographs; ESI-IT-MS, Electrospray ionization Ion-Trap Mass spectrometry; GABAA, γ-aminobutyric acid type A; RC, Respiratory Control; RPR, Rhythmic and Profound Respiration; DMACA, p-dimethylaminocinnamaldehyde
• ISSN: 2214-7500; 2214-7500
• DOI: 10.1016/j.toxrep.2021.03.024

[Display omitted] •Evaluation of EMG, ECG, and respiratory records of EEOS activity in rats.•EEOS showed sedative and muscle relaxant effects, which were similar to diazepam.•Bioactive compounds from EEOS indicate potential in pharmaceutical areas. The biological and pharmacological properties of natural polyphenols of the extract of Euterpe oleracea stone (EEOS) are associated with the central nervous system (CNS). To investigate the sedative and myorelaxant activity of EEOS in vivo, this study aimed to present the myorelaxant and sedative effects of EEOS in Wistar rats using spontaneous locomotor activity and motor electrophysiology. A total of 108 animals were used in the following experiments: a) behavioral tests (n = 27); b) electromyographic recordings of skeletal muscle (n = 27); c) respiratory muscle activity recordings (n = 27); d) cardiac muscle activity recordings (n = 27). The behavioral characteristics were measured according to the latency time of onset, the transient loss of posture reflex and maximum muscle relaxation. Electrodes were implanted in the gastrocnemius muscle and in the tenth intercostal space for electromyographic (EMG) signal capture to record muscle contraction, and in the D2 lead for electrocardiogram acquisition. After using the 300 mg/kg dose of EEOS intraperitoneally, a myorelaxant activity exhibited a lower frequency of contractility with an amplitude pattern of low and short duration at gastrocnemius muscle and intercostal muscle, which clearly describes a myorelaxant activity and changes in cardiac activity. The present report is so far the first study to demonstrate the myorelaxant activity of this extract, indicating an alternative route for açai stone valorization and its application in pharmaceutical fields.