Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production

• Published in: Cell reports (Cambridge) Vol. 29; no. 11; pp. 3394 - 3404.e9
• Main Authors: Gassaway, Brandon M., Cardone, Rebecca L., Padyana, Anil K., Petersen, Max C., Judd, Evan T., Hayes, Sebastian, Tong, Shuilong, Barber, Karl W., Apostolidi, Maria, Abulizi, Abudukadier, Sheetz, Joshua B., Kshitiz, Aerni, Hans R., Gross, Stefan, Kung, Charles, Samuel, Varman T., Shulman, Gerald I., Kibbey, Richard G., Rinehart, Jesse
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 10.12.2019; Elsevier
• Subjects: Animals; Cell Line, Tumor; Cells, Cultured; Cyclic AMP-Dependent Protein Kinases - metabolism; cyclin-dependent kinase; Cyclin-Dependent Kinases - metabolism; Diet, High-Fat; enzyme regulation; Gluconeogenesis; Glucose - metabolism; hepatic glucose production; Hepatocytes - metabolism; Insulin Resistance; Male; metabolism; nuclear localization; Phosphorylation; pyruvate kinase; Pyruvate Kinase - chemistry; Pyruvate Kinase - metabolism; Rats; Rats, Sprague-Dawley; Signal Transduction; sub-cellular localization; sub-cellular localization; hepatic glucose production; enzyme regulation; pyruvate kinase; nuclear localization; metabolism; cyclin-dependent kinase; phosphorylation; insulin resistance

Pyruvate kinase is an important enzyme in glycolysis and a key metabolic control point. We recently observed a pyruvate kinase liver isoform (PKL) phosphorylation site at S113 that correlates with insulin resistance in rats on a 3 day high-fat diet (HFD) and suggests additional control points for PK...