Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production

• Published in: Cell reports (Cambridge) Vol. 29; no. 11; pp. 3394 - 3404.e9
• Main Authors: Gassaway, Brandon M., Cardone, Rebecca L., Padyana, Anil K., Petersen, Max C., Judd, Evan T., Hayes, Sebastian, Tong, Shuilong, Barber, Karl W., Apostolidi, Maria, Abulizi, Abudukadier, Sheetz, Joshua B., Kshitiz, Aerni, Hans R., Gross, Stefan, Kung, Charles, Samuel, Varman T., Shulman, Gerald I., Kibbey, Richard G., Rinehart, Jesse
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 10.12.2019; Elsevier
• Subjects: Animals; Cell Line, Tumor; Cells, Cultured; Cyclic AMP-Dependent Protein Kinases - metabolism; cyclin-dependent kinase; Cyclin-Dependent Kinases - metabolism; Diet, High-Fat; enzyme regulation; Gluconeogenesis; Glucose - metabolism; hepatic glucose production; Hepatocytes - metabolism; Insulin Resistance; Male; metabolism; nuclear localization; Phosphorylation; pyruvate kinase; Pyruvate Kinase - chemistry; Pyruvate Kinase - metabolism; Rats; Rats, Sprague-Dawley; Signal Transduction; sub-cellular localization; sub-cellular localization; hepatic glucose production; enzyme regulation; pyruvate kinase; nuclear localization; metabolism; cyclin-dependent kinase; phosphorylation; insulin resistance
• ISSN: 2211-1247; 2211-1247
• DOI: 10.1016/j.celrep.2019.11.009

Pyruvate kinase is an important enzyme in glycolysis and a key metabolic control point. We recently observed a pyruvate kinase liver isoform (PKL) phosphorylation site at S113 that correlates with insulin resistance in rats on a 3 day high-fat diet (HFD) and suggests additional control points for PKL activity. However, in contrast to the classical model of PKL regulation, neither authentically phosphorylated PKL at S12 nor S113 alone is sufficient to alter enzyme kinetics or structure. Instead, we show that cyclin-dependent kinases (CDKs) are activated by the HFD and responsible for PKL phosphorylation at position S113 in addition to other targets. These CDKs control PKL nuclear retention, alter cytosolic PKL activity, and ultimately influence glucose production. These results change our view of PKL regulation and highlight a previously unrecognized pathway of hepatic CDK activity and metabolic control points that may be important in insulin resistance and type 2 diabetes. [Display omitted] •High-fat diet induces, whereas PKCε-knockdown rescues hepatic PKL S113 phosphorylation•PKL phosphorylation at S113 by CDKs or S12 by PKA increases gluconeogenesis•Neither S12 or S113 phosphorylation alters PKL kinetics or structure•CDK1-mediated phosphorylation of PKL S113 traps PKL in the nucleus Gassaway et al. identify a diet-induced, cyclin-dependent kinase-regulated phosphorylation site at S113 on pyruvate kinase. Although they determine that neither phosphorylation of this site nor the canonical PKA-regulated S12 site directly impacts enzyme kinetics, they demonstrate that S113 phosphorylation alters pyruvate kinase subcellular localization and influences glucose production.