Triple S-Phase Labeling of Dividing Stem Cells
Marking replicating DNA with multiple labels presents the possibility of revealing new features and mechanisms of DNA synthesis and cell division; however, progression beyond double labeling has been hampered by cross-reactivity of label detection and scarcity of appropriate labels. Here, we present...
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Published in | Stem cell reports Vol. 10; no. 2; pp. 615 - 626 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
13.02.2018
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Marking replicating DNA with multiple labels presents the possibility of revealing new features and mechanisms of DNA synthesis and cell division; however, progression beyond double labeling has been hampered by cross-reactivity of label detection and scarcity of appropriate labels. Here, we present a method for triple S-phase labeling of the dividing cells, with a fourth label used to mark cells actively engaged in cell-cycle progression (e.g., using Ki67) or to phenotype the dividing cells or their progeny (e.g., using a GFP-expressing lineage reporter transgene). We apply this method to determine the parameters of neural stem cell division in the adult brain, to birth date up to four cohorts of dividing cells, and to reveal patterns of stem cell division in non-neural tissues.
•Marking replicating DNA is now possible with three thymidine analogs•Triple S-phase labeling allows for multiple birth dating of dividing cells•Triple S-phase labeling increases resolution of cell-proliferation kinetic analysis•Triple S-phase labeling allows for combination of different labeling paradigms
In this article, Enikolopov and colleagues describe a method for triple S-phase labeling of stem cells, with an additional channel used to phenotype the cells or to add the fourth marker of cell division. They demonstrate the method's utility for birth dating multiple stem cell populations and for revealing patterns of stem cell division in the brain, testis, and intestine. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2213-6711 2213-6711 |
DOI: | 10.1016/j.stemcr.2017.12.020 |