Mycoremediation of reactive red HE7B dye by Aspergillus salinarus isolated from textile effluents

• Published in: Current research in microbial sciences Vol. 2; p. 100056
• Main Authors: Sheam, Md. Moinuddin, Biswas, Sudhangshu Kumar, Ahmed, Kazi Rejvee, Syed, Shifath Bin, Hossain, Md. Shamim, Khan, Md. Shakil Ahmed, Hasan, Md. Rockybul, Zohra, Fatema Tuj, Rahman, Md. Mizanur
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2021; Elsevier
• Subjects: Aspergillus; Bioremediation; Decolorization; Fungi; Research Paper; Textile dye; Fungi; Aspergillus; Decolorization; Bioremediation; Textile dye
• ISSN: 2666-5174; 2666-5174
• DOI: 10.1016/j.crmicr.2021.100056

•Annually, 280,000 tons of clothing dyes are discharged into industrial effluents.•Such synthetic dyes are harmful to the environment and to people's health.•Bioremediation of these dyes are sustainable and eco-friendly approach.•Three RR dye degrading fungal stains had been identified by ITS sequencing.•These Aspergillus salinarus stains can degrade up to 97.41% RR dye (50 mg/L). Reactive dyes are widely utilized in the textile industry due to their advantageous properties of vivid color, water-fastness, and simple application procedures with minimal energy usage. The toxicity of most azo dyes is a significant environmental concern, as effluents from dye processing and manufacturing sectors are known to be carcinogenic and mutagenic to numerous species. These issues are more grievous in Bangladesh, one of the largest exporters of apparel. This study aimed to isolate and identify potential fungal strains from textile effluent that are capable of degrading Reactive Red HE7B dye (a sulphonated reactive azo dye), a widely used dye in local thread dyeing industries. Dye degradation assay was performed in potato dextrose broth supplemented with 50 mg/l Reactive Red HE7B and the degradation rate was measured by a UV spectrophotometer. DNA extraction, quantification, PCR, internal transcribed spacer (ITS) sequencing, and phylogenetic analysis were performed to identify the selected fungi. Among the isolates, the three best performing strains TEF -3, TEF -4, and TEF -5 showed 97.41%, 93.12%, and 82.89% dye degrading efficacy after 96 h of incubation, respectively. All three strains, TEF-3, TEF-4, and TEF-5 showed similarity with Aspergillus salinarus (accession no. NR_157473.1) and the similarity percentages were 97.02, 96.95, and 95.28 respectively. Interestingly, this study probably the very first indication of textile dye degradation by Aspergillus salinarus strains. Thus, these fungal strains possess the prospectiveness to be utilized in the textile wastewater treatment plants, since the isolates demonstrated the substantial capacity (>80%) to degrade Reactive Red dye after 96 h of incubation. [Display omitted]