Metal Binding Specificity of the MntABC Permease of Neisseria gonorrhoeae and Its Influence on Bacterial Growth and Interaction with Cervical Epithelial Cells

• Published in: Infection and Immunity Vol. 76; no. 8; pp. 3569 - 3576
• Main Authors: Lim, Karen H.L, Jones, Christopher E, vanden Hoven, Rachel N, Edwards, Jennifer L, Falsetta, Megan L, Apicella, Michael A, Jennings, Michael P, McEwan, Alastair G
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.08.2008; American Society for Microbiology (ASM)
• Subjects: Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Binding Sites; Biofilms - growth & development; Biological and medical sciences; Cations, Divalent - metabolism; Cell Line; Cervix Uteri - cytology; Cervix Uteri - microbiology; Epithelial Cells - microbiology; Female; Fundamental and applied biological sciences. Psychology; Humans; Kinetics; Membrane Transport Proteins - genetics; Membrane Transport Proteins - metabolism; Metals - metabolism; Microbiology; Miscellaneous; Molecular Pathogenesis; Neisseria gonorrhoeae; Neisseria gonorrhoeae - enzymology; Neisseria gonorrhoeae - genetics; Neisseria gonorrhoeae - growth & development; Neisseria gonorrhoeae - pathogenicity; Periplasmic Binding Proteins - genetics; Periplasmic Binding Proteins - metabolism; Specificity; Neisseria gonorrhoeae; Microbiology; Permease; Neisseriaceae; Bacteria; Micrococcales; Epithelial cell; Immunity
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.01725-07

mntABC from Neisseria gonorrhoeae encodes an ABC permease which includes a periplasmic divalent cation binding receptor protein of the cluster IX family, encoded by mntC. Analysis of an mntC mutant showed that growth of N. gonorrhoeae could be stimulated by addition of either manganese(II) or zinc(II) ions, suggesting that the MntABC system could transport both ions. In contrast, growth of the mntAB mutant in liquid culture was possible only when the medium was supplemented with an antioxidant such as mannitol, consistent with the view that ion transport via MntABC is essential for protection of N. gonorrhoeae against oxidative stress. Using recombinant MntC, we determined that MntC binds Zn²⁺ and Mn²⁺ with almost equal affinity (dissociation constant of ~0.1 μM). Competition assays with the metallochromic zinc indicator 4-(2-pyridylazo)resorcinol showed that MntC binds Mn²⁺ and Zn²⁺ at the same binding site. Analysis of the N. gonorrhoeae genome showed that MntC is the only Mn/Zn metal binding receptor protein cluster IX in this bacterium, in contrast to the situation in many other bacteria which have systems with dedicated Mn and Zn binding proteins as part of distinctive ABC cassette permeases. Both the mntC and mntAB mutants had reduced intracellular survival in a human cervical epithelial cell model and showed reduced ability to form a biofilm. These data suggest that the MntABC transporter is of importance for survival of Neisseria gonorrhoeae in the human host.