Cell culture of small round cell tumor originating in the thoracopulmonary region. Evidence for derivation from a primitive pluripotent cell

• Published in: Cancer Vol. 64; no. 1; pp. 43 - 51
• Main Authors: Fujii, Yuji, Hongo, Teruaki, Nakagawa, Yuichi, Nasuda, Kaoru, Mizuno, Yoshihito, Igarashi, Yoshio, Naito, Yasuhisa, Maeda, Matsuyoshi
• Format: Journal Article
• Language: English
• Published: New York Wiley Subscription Services, Inc., A Wiley Company 01.07.1989
• Subjects: Adolescent; Animals; Carcinoembryonic Antigen - analysis; DNA, Neoplasm - analysis; Female; Humans; Mice; Mice, Nude; Neoplastic Stem Cells; Oncogenes; Phosphopyruvate Hydratase - analysis; Thoracic Neoplasms - genetics; Thoracic Neoplasms - metabolism; Thoracic Neoplasms - pathology; Translocation, Genetic; Tumor Cells, Cultured
• ISSN: 0008-543X; 1097-0142
• DOI: 10.1002/1097-0142(19890701)64:1<43::AID-CNCR2820640109>3.0.CO;2-F

The authors describe a 14‐year‐old girl with small round cell tumor originating in the chest wall analyzed by the extensive studies including light and electron microscopic examination, histochemical study, immunochemical study, cytogenetics, and gene analysis. A cell line producing carcinoembryonic antigen (CEA) and neuron‐specific enolase (NSE) has been established from pleural effusion of the pulmonary metastatic tumor. Cytogenetic analysis disclosed a reciprocal translocation (11;22)(q24;q12). Additionally, immunocytochemical studies demonstrated that CEA, NSE, vimentin, cytokeratin, and epithelial membrane antigens are positive, but desmin and S‐100 protein are negative. Although neurofilament was negative in the pulmonary metastatic tumor cells, it became positive in cell line in vitro. These results suggest that this tumor may be derived from the primitive and pluripotential cells, differentiating into mesenchymal, epithelial, and neural features in variable proportions.