T Cell Membrane Mimicking Nanoparticles with Bioorthogonal Targeting and Immune Recognition for Enhanced Photothermal Therapy

• Published in: Advanced science Vol. 6; no. 15; pp. 1900251 - n/a
• Main Authors: Han, Yutong, Pan, Hong, Li, Wenjun, Chen, Ze, Ma, Aiqing, Yin, Ting, Liang, Ruijing, Chen, Fuming, Ma, Yifan, Jin, Yan, Zheng, Mingbin, Li, Baohong, Cai, Lintao
• Format: Journal Article
• Language: English
• Published: Germany John Wiley & Sons, Inc 07.08.2019; John Wiley and Sons Inc; Wiley
• Subjects: biomimetic nanoparticles; bioorthogonal chemistry; Lasers; Lymphocytes; Medical research; Membranes; Metabolism; Nanomaterials; Nanoparticles; photothermal therapy; Proteins; Scientific imaging; T cell membranes; T cell receptors; Temperature; tumor dual targeting; Tumors; United States > US; biomimetic nanoparticles; bioorthogonal chemistry; T cell membranes; tumor dual targeting; photothermal therapy
• ISSN: 2198-3844; 2198-3844
• DOI: 10.1002/advs.201900251

Due to specific immune recognition receptors on the surface of T cells, their membranes are promising mimic nanocarriers for delivering drugs to tumor lesions. However, this single targeting strategy potentially compromises therapy efficacy for tumor targeting due to inter‐ and intra‐heterogeneity of tumors. Azide (N3) or bicyclo [6.1.0] nonyne (BCN) modified unnatural sugars can be successfully incorporated into surface glycans of various tumor cells as artificial receptors, which is expected to overcome the insufficiency of single targeting. Based on this artificial tumor targeting strategy, indocyanine green nanoparticles (INPs) coated with N3‐labeled T cell membrane (N3‐TINPs) are constructed, which can specifically target the natural antigen and BCN artificial receptors on tumors through immune recognition and bioorthogonal chemistry, respectively. The results show that the fluorescence intensity in the tumors of mice treated with N3‐TINPs is 1.5 fold compared with that of the mice treated with unlabeled TINPs. The accumulated N3‐TINPs in the tumor significantly increase the photothermal therapeutic effect without adverse effect. Therefore, this T cell membrane mimicking nanoparticles based bioorthogonal chemistry may provide an alternative artificial targeting strategy for further tumor targeting photothermal therapy. The biomimetic nanoparticles (N3‐TINPs) consisting of an indocyanine green (ICG) polymeric core and azide (N3)‐labeled T cell membrane shell, exhibit highly effective tumor targeting and elimination effect. N3‐TINPs can effectively anchor tumor cells by a dual‐targeting mechanism, namely immune recognition of the T cell membrane and N3‐BCN bioorthogonal targeting, and exhibit a highly efficient ICG‐mediated photothermal therapeutic effect.