Complex extracellular interactions of proteases and a protease inhibitor influence multicellular development of Streptomyces coelicolor

• Published in: Molecular microbiology Vol. 70; no. 5; pp. 1180 - 1193
• Main Authors: Kim, Dae Wi, Hesketh, Andy, Kim, Eun Sook, Song, Ju Yeon, Lee, Dae Hoon, Kim, In Seop, Chater, Keith F, Lee, Kye Joon
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.12.2008; Blackwell Publishing Ltd; Blackwell
• Subjects: Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Biological and medical sciences; DNA, Complementary - genetics; Eukaryotes; Fermentation; Fundamental and applied biological sciences. Psychology; Gene Deletion; Gene Expression Regulation, Bacterial; Growth, nutrition, cell differenciation; Microbiology; Mutation; Peptide Hydrolases - metabolism; Protease inhibitors; Protease Inhibitors - metabolism; Protein Processing, Post-Translational; Ribonucleic acid; RNA; RNA, Bacterial - genetics; RNA, Transfer, Leu - metabolism; Streptomyces; Streptomyces coelicolor; Streptomyces coelicolor - genetics; Streptomyces coelicolor - growth & development; Streptomyces coelicolor - metabolism; Two-Hybrid System Techniques; Streptomycetaceae; Microbiology; Streptomyces coelicolor; Molecular interaction; Actinomycetales; Regulation(control); Development; Bacteria; Antiviral; Colony; Actinomycetes; Models; Protease inhibitor
• ISSN: 0950-382X; 1365-2958
• DOI: 10.1111/j.1365-2958.2008.06471.x

Streptomyces coelicolor produces an extracellular protease inhibitor protein, STI (Streptomyces trypsin inhibitor). We show that post-growth elimination of STI is needed for colonies to develop aerial mycelium efficiently. Inactivation of STI, and thus the normal progression of colony development, at least partly involves an extracellular protease specified by gene SCO5913. Two-hybrid analysis identified two possible targets of STI inhibition (the products of SCO1355 and SCO5447), both extracellular proteases containing a domain homologous with the P-domain of eukaryotic convertases, proteases that mediate the processing of many precursors with important cellular or developmental roles. At least the SCO1355 protease is needed for the normal progression of development. Two components of the proposed cascade are dependent on the tRNA for the rare UUA (leucine) codon, which is specified by the developmental gene bldA. A model is presented that links intracellular regulatory events with an extracellular protease cascade to facilitate normal development.