Polymorphic microsatellite loci in the widespread amphidromous goby Sicyopterus lagocephalus and cross-genus amplification among Sicydiinae

• Published in: Molecular ecology resources Vol. 9; no. 2; pp. 607 - 609
• Main Authors: HOAREAU, T.B, BARBISAN, F, DUBOIS, S, ZANE, L, BERREBI, P
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.03.2009; Blackwell Publishing Ltd; Wiley/Blackwell
• Subjects: amphidromous gobiids; Biodiversity and Ecology; cross-genus amplification; domain_sde.be.gp; Environmental Sciences; Genetic markers; microsatellite; microsatellite repeats; Sicydiinae; Sicydiins; Sicyopterus lagocephalus; Wahlund effect; microsatellite; Genetic markers; Wahlund effect; Sicydiins; amphidromous gobiids; cross-genus amplification
• ISSN: 1755-0998; 1755-098X; 1755-0998
• DOI: 10.1111/j.1755-0998.2008.02461.x

Microsatellite DNA markers were isolated in an amphidromous goby (Sicyopterus lagocephalus) from a partial genomic library enriched for AC repeats. Eight microsatellites were highly polymorphic with six to 33 alleles per locus and expected heterozygosities ranging from 0.53 to 0.97. Cross-species amplifications were performed within the sub-family Sicydiinae by genotyping individuals from two species of the genus Cotylopus. Some of these loci were successfully amplified and showed polymorphism in the second genus.