Comparison of the oral microbiome of siblings using next-generation sequencing: a pilot study

• Published in: Oral diseases Vol. 22; no. 6; pp. 549 - 556
• Main Authors: Lee, H-S, Lee, JH, Kim, S-O, Song, JS, Kim, BI, Kim, YJ, Lee, J-H
• Format: Journal Article
• Language: English
• Published: Denmark Blackwell Publishing Ltd 01.09.2016; Wiley Subscription Services, Inc
• Subjects: Dental caries; Dental Caries - microbiology; Humans; Microbiology; Mouth - microbiology; next-generation sequencing; Oral diseases; oral microbiome; Pilot Projects; pyrosequencing; sibling; Siblings; dental caries; oral microbiome; pyrosequencing; sibling; next-generation sequencing
• ISSN: 1354-523X; 1601-0825; 1601-0825
• DOI: 10.1111/odi.12491

Objective The purpose of this study was to compare the oral microbiome of siblings with and without dental caries using next‐generation sequencing. Materials and Methods To investigate the oral microbiome composition, 14 young siblings, seven with caries and seven without, were enrolled from seven sibling‐pair families. Supragingival plaque samples were collected from the cervicobuccal area of posterior teeth. All samples were analyzed by pyrosequencing, based on the 16S rRNA gene hypervariable regions, V1–V4. Results The organisms identified belonged to 65 genera. Fifty‐two genera were identified in the subjects with caries and 58 in those without; 45 genera were shared by both groups. In the Shannon index, the caries group showed lower bacterial diversity than the caries‐free group and the difference was significant (Wilcoxon signed‐rank test, P < 0.05). Additionally, similarities between siblings were evident in analyses based on weighted UniFrac distances (P < 0.05). Conclusions In this study, the diversity of the microbiome was reduced in subjects with dental caries, while similarity between siblings seemed to be retained.