Functional analysis of the C-terminal region of the vacuolar cadmium-transporting rice OsHMA3

•The C-terminal domain of OsHMA3 is essential for the transport of Cd in plants.•The first 105aa of the C-terminal region have important roles in OsHMA3 function.•The C-terminal domain is not involved in the subcellular localization of OsHMA3.•The cysteine pair is not necessary for Cd transport in p...

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Published inFEBS letters Vol. 588; no. 5; pp. 789 - 794
Main Authors Kumagai, Saori, Suzuki, Tatsuya, Tezuka, Kouichi, Satoh-Nagasawa, Namiko, Takahashi, Hidekazu, Sakurai, Kenji, Watanabe, Akio, Fujimura, Tatsuhito, Akagi, Hiromori
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 03.03.2014
Subjects
Adenosine Triphosphatases - chemistry
Adenosine Triphosphatases - physiology
Amino Acid Substitution
Arabidopsis
Biological Transport
C-terminal domain
Cadmium
Cadmium - metabolism
Cation Transport Proteins - chemistry
Cation Transport Proteins - physiology
Mutagenesis, Site-Directed
Onions
Oryza - enzymology
OsHMA3
P1B-ATPase (HMA)
Plant Epidermis - enzymology
Plant Proteins - chemistry
Plant Proteins - physiology
Protein Structure, Tertiary
Protein Transport
Rice
Vacuolar sequestration
Vacuoles - enzymology
P1B-ATPase (HMA)
C-terminal domain
Cadmium
OsHMA3
Vacuolar sequestration
Rice
P(1B)-ATPase (HMA)
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Summary:•The C-terminal domain of OsHMA3 is essential for the transport of Cd in plants.•The first 105aa of the C-terminal region have important roles in OsHMA3 function.•The C-terminal domain is not involved in the subcellular localization of OsHMA3.•The cysteine pair is not necessary for Cd transport in plants. Rice OsHMA3 is a vacuolar cadmium (Cd) transporter belonging to the P1B-ATPase family and has a long (273aa) C-terminal region. We analyzed the function of the region related to Cd using the transgenic Arabidopsis Col-0 ecotype, which is sensitive to Cd. The OsHMA3 variant containing a truncated (58aa) C-terminal region did not confer Cd tolerance, whereas an OsHMA3 variant containing a longer truncated (105aa) C-terminal region conferred Cd tolerance to transgenic Arabidopsis. We conclude that the C-terminal region, particularly the region containing the first 105aa, has an important role in OsHMA3 activity.
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content type line 23
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2014.01.037