Tricin inhibits CCL5 induction required for the efficient growth of human cytomegalovirus

It has been reported that treatment of human embryonic lung fibroblast (HEL) cells with tricin (4', 5, 7-trihydroxy-3', 5'-dimethoxyflavone) following infection by human cytomegalovirus (HCMV) significantly suppresses HCMV replication. In the present work, we examined the mechanisms f...

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Published inMicrobiology and immunology
Main Authors Itho, Akimasa, Sadanari, Hidetaka, Takemoto, Masaya, Matsubara, Keiko, Daikoku, Tohru, Murayama, Tsugiya
Format Journal Article
LanguageEnglish
Published Australia 01.05.2018
Subjects
agent
Tricin
CCL5
Anti-cytomegalovirus
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Summary:It has been reported that treatment of human embryonic lung fibroblast (HEL) cells with tricin (4', 5, 7-trihydroxy-3', 5'-dimethoxyflavone) following infection by human cytomegalovirus (HCMV) significantly suppresses HCMV replication. In the present work, we examined the mechanisms for the anti-HCMV effects of tricin in HEL cells. Exposure of HEL cells to tricin inhibited HCMV replication, with concomitant decreases in transcripts levels of the CC chemokine RANTES (CCL5) - encoding gene and in the expression of the CCL5 protein. We also found that transcripts of HCMV immediate early 1 (IE1), and HCMV UL54 (encoding DNA polymerase), and replication of HCMV was significantly lower in CCL5 gene-knockdown cells. These results suggested that tricin has different anti-HCMV activity from ganciclovir, and that CCL5 is one of the chemokines involved in HCMV replication. In addition, it is possible that chemokine CCL5 is one of the targets of tricin.
ISSN:1348-0421
DOI:10.1111/1348-0421.12590