The N-Terminal Region of Plasmodium falciparum MSP10 Is a Target of Protective Antibodies in Malaria and Is Important for PfGAMA/PfMSP10 Interaction
Clinical manifestation of malaria is mainly due to intra-erythrocytic development of parasites. merozoites, the invasive form of the blood-stage parasite, invade human erythrocytes in a complex but rapid process. This multi-step progression involves interactions between parasite and human host prote...
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Published in | Frontiers in immunology Vol. 10; p. 2669 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
20.11.2019
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Subjects | |
Online Access | Get full text |
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Summary: | Clinical manifestation of malaria is mainly due to intra-erythrocytic development of
parasites.
merozoites, the invasive form of the blood-stage parasite, invade human erythrocytes in a complex but rapid process. This multi-step progression involves interactions between parasite and human host proteins. Here we show that antibodies against a vaccine antigen, PfGAMA, co-immunoprecipitate with PfMSP10. This interaction was validated as direct by surface plasmon resonance analysis. We then demonstrate that antibodies against PfMSP10 have growth inhibitory activity against cultured parasites, with the region PfMSP10 R1 that is critical for its interaction with PfGAMA being the key target. We also observe that the PfMSP10 R1 region is highly conserved among African field isolates. Lastly, we show that high levels of antibodies against PfMSP10 R1 associate with reduced risk to clinical malaria in children resident in a malaria endemic region in northern Uganda. Put together, these findings provide for the first time the functional context of the important role of PfGAMA/PfMSP10 interaction in erythrocyte invasion and unveil a novel asexual blood-stage malaria vaccine target for attenuating
merozoite invasion. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Microbial Immunology, a section of the journal Frontiers in Immunology Present address: Thangavelu U. Arumugam, Department of Biotechnology, Faculty Center for Agricultural Education and Research, Ramakrishna Mission Vivekananda Educational and Research Institute, Coimbatore, India Reviewed by: Julius Clemence Hafalla, University of London, United Kingdom; Giampietro Corradin, Université de Lausanne, Switzerland Edited by: Amy Rasley, United States Department of Energy (DOE), United States |
ISSN: | 1664-3224 1664-3224 |
DOI: | 10.3389/fimmu.2019.02669 |