Anthrax Toxin Triggers Endocytosis of Its Receptor via a Lipid Raft-Mediated Clathrin-Dependent Process
The protective antigen (PA) of the anthrax toxin binds to a cell surface receptor and thereby allows lethal factor (LF) to be taken up and exert its toxic effect in the cytoplasm. Here, we report that clustering of the anthrax toxin receptor (ATR) with heptameric PA or with an antibody sandwich caus...
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Published in | The Journal of cell biology Vol. 160; no. 3; pp. 321 - 328 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Rockefeller University Press
03.02.2003
The Rockefeller University Press |
Subjects | |
Online Access | Get full text |
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Summary: | The protective antigen (PA) of the anthrax toxin binds to a cell surface receptor and thereby allows lethal factor (LF) to be taken up and exert its toxic effect in the cytoplasm. Here, we report that clustering of the anthrax toxin receptor (ATR) with heptameric PA or with an antibody sandwich causes its association to specialized cholesterol and glycosphingolipid-rich microdomains of the plasma membrane (lipid rafts). We find that although endocytosis of ATR is slow, clustering it into rafts either via PA heptamerization or using an antibody sandwich is necessary and sufficient to trigger efficient internalization and allow delivery of LF to the cytoplasm. Importantly, altering raft integrity using drugs prevented LF delivery and cleavage of cytosolic MAPK kinases, suggesting that lipid rafts could be therapeutic targets for drugs against anthrax. Moreover, we show that internalization of PA is dynamin and Eps15 dependent, indicating that the clathrin-dependent pathway is the major route of anthrax toxin entry into the cell. The present work illustrates that although the physiological role of the ATR is unknown, its trafficking properties, i.e., slow endocytosis as a monomer and rapid clathrin-mediated uptake on clustering, make it an ideal anthrax toxin receptor. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 Address correspondence to F. Gisou van der Goot, Dept. of Genetics and Microbiology, 1 rue Michel Servet, 1211 Geneva 4, Switzerland. Tel.: (41) 022-702-5652. Fax: (41) 022-702-5896. E-mail: gisou.vandergoot@medecine.unige.ch |
ISSN: | 0021-9525 1540-8140 |
DOI: | 10.1083/jcb.200211018 |