Efficient CRISPR-Cas9 Gene Disruption System in Edible-Medicinal Mushroom Cordyceps militaris

is a well-known edible medicinal mushroom in East Asia that contains abundant and diverse bioactive compounds. Since traditional genome editing systems in were inefficient and complicated, here, we show that the codon-optimized , which was used with the newly reported promoter Pcmlsm3 and terminator...

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Published inFrontiers in microbiology Vol. 9; p. 1157
Main Authors Chen, Bai-Xiong, Wei, Tao, Ye, Zhi-Wei, Yun, Fan, Kang, Lin-Zhi, Tang, Hong-Biao, Guo, Li-Qiong, Lin, Jun-Fang
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 12.06.2018
Subjects
Cordyceps militaris
CRISPR-Cas9
edible-medicinal mushroom
fungi industry
gene disruption
Microbiology
Cordyceps militaris
CRISPR-Cas9
gene disruption
fungi industry
edible-medicinal mushroom
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Summary:is a well-known edible medicinal mushroom in East Asia that contains abundant and diverse bioactive compounds. Since traditional genome editing systems in were inefficient and complicated, here, we show that the codon-optimized , which was used with the newly reported promoter Pcmlsm3 and terminator Tcmura3, was expressed. Furthermore, with the help of the negative selection marker , a CRISPR-Cas9 system that included the Cas9 DNA endonuclease, RNA presynthesized and a single-strand DNA template efficiently generated site-specific deletion and insertion. This is the first report of a CRISPR-Cas9 system in , and it could accelerate the genome reconstruction of to meet the need for rapid development in the fungi industry.
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This article was submitted to Fungi and Their Interactions, a section of the journal Frontiers in Microbiology
Edited by: Hector Mora Montes, Universidad de Guanajuato, Mexico
Reviewed by: Patricia Ponce-Noyola, Universidad de Guanajuato, Mexico; Attila Gacser, University of Szeged, Hungary
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2018.01157