Actin Turnover Is Required to Prevent Axon Retraction Driven by Endogenous Actomyosin Contractility
Growth cone motility and guidance depend on the dynamic reorganization of filamentous actin (F-actin). In the growth cone, F-actin undergoes turnover, which is the exchange of actin subunits from existing filaments. However, the function of F-actin turnover is not clear. We used jasplakinolide (jasp...
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Published in | The Journal of cell biology Vol. 158; no. 7; pp. 1219 - 1228 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
Rockefeller University Press
30.09.2002
The Rockefeller University Press |
Subjects | |
Online Access | Get full text |
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Summary: | Growth cone motility and guidance depend on the dynamic reorganization of filamentous actin (F-actin). In the growth cone, F-actin undergoes turnover, which is the exchange of actin subunits from existing filaments. However, the function of F-actin turnover is not clear. We used jasplakinolide (jasp), a cell-permeable macrocyclic peptide that inhibits F-actin turnover, to study the role of F-actin turnover in axon extension. Treatment with jasp caused axon retraction, demonstrating that axon extension requires F-actin turnover. The retraction of axons in response to the inhibition of F-actin turnover was dependent on myosin activity and regulated by RhoA and myosin light chain kinase. Significantly, the endogenous myosin-based contractility was sufficient to cause axon retraction, because jasp did not alter myosin activity. Based on these observations, we asked whether guidance cues that cause axon retraction (ephrin-A2) inhibit F-actin turnover. Axon retraction in response to ephrin-A2 correlated with decreased F-actin turnover and required RhoA activity. These observations demonstrate that axon extension depends on an interaction between endogenous myosin-driven contractility and F-actin turnover, and that guidance cues that cause axon retraction inhibit F-actin turnover. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 Address correspondence to Gianluca Gallo at his present address, Dept. of Neurobiology and Anatomy, Drexel Univ. College of Medicine, 2900 Queen Ln., Philadelphia, PA 19129. Tel.: (215) 991-8288. Fax: (215) 843-9082. E-mail: Neurite@aol.com |
ISSN: | 0021-9525 1540-8140 |
DOI: | 10.1083/jcb.200204140 |