Proteomics studies of post-translational modifications in plants

• Published in: Journal of experimental botany Vol. 57; no. 7; pp. 1547 - 1551
• Main Authors: Kwon, Sun Jae, Choi, Eun Young, Choi, Yoon Jung, Ahn, Ji Hoon, Park, Ohkmae K
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Oxford Oxford University Press 01.04.2006; Oxford Publishing Limited (England)
• Subjects: amino acid sequences; Biochemistry; Biological and medical sciences; Cell biochemistry; Cell membranes; Cell physiology; Chromatography; Chromatography, Affinity; Fundamental and applied biological sciences. Psychology; Gels; Genomics; Glycosylphosphatidylinositols - metabolism; Immunoprecipitation; literature reviews; mass spectrometry; molecular sequence data; new methods; Phosphorylation; physiological regulation; Plant physiology and development; Plant Proteins - metabolism; Plants; Post translational modification; Protein Processing, Post-Translational; protein structure; protein synthesis; Proteomics; Proteomics - methods; sample preparation; sampling; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Thylakoids; Ubiquitin - metabolism; ubiquitination; Proteomics; plant; Development; Glycosylphosphatidylinositol; Review; post-translational modification; phosphorylation
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/erj137

Post-translational modifications of proteins greatly increase protein complexity and dynamics, co-ordinating the intricate regulation of biological events. The global identification of post-translational modifications is a difficult task that is currently accelerated by advances in proteomics techniques. There has been significant development in sample preparation methods and mass spectrometry instrumentation. To reduce the complexity and to increase the amount of modified proteins available for analysis, proteins are usually subjected to prefractionation such as chromatographic purification and affinity enrichment. In this review, the post-translational modification studies in plants are summarized. The sample preparation strategies applied to each study are also described. These include affinity-based enrichment methods, immobilized metal affinity chromatography and immunoprecipitation used for phosphorylation and ubiquitination studies, respectively, and the phase partitioning approach for glycosylphosphatidylinositol modification studies.