Photosynthetic electron transport controls expression of the high light inducible gene in the cyanobacterium Synechococcus elongatus strain PCC 7942

• Published in: Plant and cell physiology Vol. 45; no. 5; pp. 651 - 658
• Main Authors: Salem, K. (University of Texas at Arlington (USA)), van Waasbergen, L.G
• Format: Journal Article
• Language: English
• Published: Japan Oxford University Press 01.05.2004; Oxford Publishing Limited (England)
• Subjects: 1-dimethylurea; 4-dichlorophenyl)-1; 5-dibromo-3-methyl-6-isopropyl-benzoquinone; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacterial Proteins - radiation effects; blue/UV-A light; Cell Respiration - genetics; Cell Respiration - radiation effects; CYANOBACTERIA; Cyanobacteria - genetics; Cyanobacteria - metabolism; Cyanobacteria - radiation effects; Cyanophyta; Cytochrome b6f Complex - metabolism; DBMIB; DCMU; Dibromothymoquinone - pharmacology; Diuron - pharmacology; Electron Transport - genetics; Electron Transport - radiation effects; GENE EXPRESSION; Gene Expression Regulation, Bacterial - genetics; Gene Expression Regulation, Bacterial - radiation effects; high light; Keywords: Blue/UV-A light — Cyanobacteria — Electron transport control — High intensity light — High light inducible gene — Synechococcus elongatus PCC 7942; Light; LIGHT REGIMES; Light-Harvesting Protein Complexes - genetics; Light-Harvesting Protein Complexes - metabolism; Light-Harvesting Protein Complexes - radiation effects; low light; Photic Stimulation; PHOTOSYNTHESIS; Photosynthesis - genetics; Photosynthesis - radiation effects; Potassium Cyanide - pharmacology; RNA Stability - drug effects; RNA Stability - genetics; RNA, Messenger - drug effects; RNA, Messenger - metabolism; Species Specificity; Synechococcus; Synechococcus elongatus; ULTRAVIOLET RADIATION; Up-Regulation - genetics; Up-Regulation - radiation effects
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/pcp/pch072

The hliA gene of Synechococcus, encoding a photoprotective high light inducible polypeptide, is up-regulated by high light (HL) or low intensity blue/UV-A light (BL). hliA expression was found to be up-regulated by KCN in low light (LL) (but not in the dark), and up-regulation in HL, BL, and LL (with KCN) was inhibited by 2,5-dibromo-3-methyl-6-isopropyl-benzoquinone. A working hypothesis is proposed whereby up-regulation is in response to the reduced state of cytochrome b6f or a carrier beyond in photosynthesis. Modest up-regulation occurs in LL by treatment with 3-(3,4-dichlorophenyl)-1,1-dimethylurea, but this is related to effects on hliA mRNA stability rather than on transcription.