Western blot analysis of virus-specific antibody responses for capripox and contagious pustular dermatitis viral infections in sheep

• Published in: Epidemiology and infection Vol. 113; no. 2; pp. 377 - 385
• Main Authors: Chand, P., Kitching, R. P., Black, D. N.
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.10.1994
• Subjects: Animals; Antibodies; Antibodies, Viral - blood; Antibodies, Viral - isolation & purification; Antigens; Antiserum; Blotting, Western; capripoxvirus; Capripoxvirus - immunology; Dermatitis; Diagnosis, Differential; Ecthyma, Contagious - diagnosis; Ecthyma, Contagious - immunology; Gels; Immunodiffusion; Infections; Inoculation; Poxviridae; Poxviridae Infections - diagnosis; Poxviridae Infections - immunology; Poxviridae Infections - veterinary; Sheep; Sheep Diseases - diagnosis; Sheep Diseases - immunology; Viruses
• ISSN: 0950-2688; 1469-4409
• DOI: 10.1017/S0950268800051803

This paper reports the development and evaluation of serological tests for the differentiation of antibodies in animals infected with capripox and parapox viruses. Agar-gel immunodiffusion tests using sera from sheep with naturally-acquired infections and from sheep experimentally inoculated with orf or capripox viruses showed cross reactions. Virus-specific antibody responses to structural proteins of the viruses were analysed by Western-blot analysis. This analysis readily differentiated the infections as either capripox or contagious pustular dermatitis. The antibody responses to the 32 kDa and 26 kDa proteins of capripox virus provided a firm basis for differention.