The levels of NF-κB p50 and NF-κB p65 play a role in thyroid carcinoma malignancy in vivo

• Published in: Journal of international medical research Vol. 46; no. 10; pp. 4092 - 4099
• Main Authors: Le, Fei, Zhang, Jing-Yu, Liu, Wei, Huang, Xian-Ming, Luo, Wen-Zheng
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.10.2018; Sage Publications Ltd; SAGE Publishing
• Subjects: Adolescent; Adult; Aged; China; Clinical Research Reports; Female; Humans; Immunohistochemistry; Lymphatic Metastasis; Lymphatic system; Male; Metastasis; Middle Aged; NF-kappa B p50 Subunit - blood; Prospective Studies; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - blood; Thyroid cancer; Thyroid Neoplasms - blood; Thyroid Neoplasms - metabolism; Thyroid Neoplasms - pathology; Transcription Factor RelA - blood; Young Adult; Thyroid cancer; nuclear factor-κB p65; immunohistochemistry; quantitative real-time reverse transcription–polymerase chain reaction; nuclear factor-κB p50
• ISSN: 0300-0605; 1473-2300
• DOI: 10.1177/0300060518785846

Background To investigate the relationship between the levels of nuclear factor (NF)-κB p50 and NF-κB p65 and tumour characteristics in patients with thyroid carcinoma. Methods This prospective study enrolled consecutive patients with thyroid carcinoma. Tumour samples were collected and the levels of NF-κB p50 and NF-κB p65 protein and mRNA were measured using immunohistochemistry and quantitative real-time reverse transcription–polymerase chain reaction (qRT–PCR). Results A total of 73 patients with thyroid carcinoma were included in the study (20 males; 53 females; mean ± SD age, 44.8 ± 12.7 years, range, 18–76 years). There were no significant differences in sex, age and pathological type between the NF-κB p50 positive group and the NF-κB p50 negative group, but tumour diameter and lymph node metastasis were significantly higher in the NF-κB p50 positive group compared with the NF-κB p50 negative group. Similar findings were observed for NF-κB p65. The levels of NF-κB p50 were positively correlated with NF-κB p65 in samples of thyroid carcinoma (rs = 0.653). Conclusion The levels of NF-κB p50 and NF-κB p65 in samples of thyroid carcinoma were positively associated with tumour diameter and the presence of lymph node metastasis.