Lower Expression of SLC27A1 Enhances Intramuscular Fat Deposition in Chicken via Down-Regulated Fatty Acid Oxidation Mediated by CPT1A
Intramuscular fat (IMF) is recognized as the predominant factor affecting meat quality due to its positive correlation with tenderness, juiciness, and flavor. Chicken IMF deposition depends on the balance among lipid synthesis, transport, uptake, and subsequent metabolism, involving a lot of genes a...
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Published in | Frontiers in physiology Vol. 8; p. 449 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
29.06.2017
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Subjects | |
Online Access | Get full text |
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Summary: | Intramuscular fat (IMF) is recognized as the predominant factor affecting meat quality due to its positive correlation with tenderness, juiciness, and flavor. Chicken IMF deposition depends on the balance among lipid synthesis, transport, uptake, and subsequent metabolism, involving a lot of genes and pathways, however, its precise molecular mechanisms remain poorly understood. In the present study, the breast muscle tissue of female Wenchang chickens (WC) (higher IMF content, 1.24 in D120 and 1.62 in D180) and female White Recessive Rock chickens (WRR; lower IMF content, 0.53 in D120 and 0.90 in D180) were subjected to RNA-sequencing (RNA-seq) analysis. Results showed that many genes related to lipid catabolism, such as
, and
were down-regulated in WC chickens, and these genes were involved in the PPAR signaling pathway and formed an IPA® network related to lipid metabolism. Furthermore,
was more down-regulated in WRR.D180.B than in WRR.D120.B. Decreased cellular triglyceride (TG) and up-regulated
were observed in the
overexpression QM-7 cells, and increased cellular triglyceride (TG) and down-regulated
were observed in the
knockdown QM-7 cells. These results suggest that lower lipid catabolism exists in WC chickens but not in WRR chickens, and lower expression of
facilitate IMF deposition in chicken
down-regulated fatty acid oxidation mediated by
. These findings indicate that reduced lipid catabolism, rather than increased lipid anabolism, contributes to chicken IMF deposition. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Lykke Sylow, University of Copenhagen, Denmark; Mildred Audrey Pointer, North Carolina Central University, United States Edited by: Leonardo Alexandre Peyré-Tartaruga, Universidade Federal do Rio Grande do Sul, Brazil This article was submitted to Integrative Physiology, a section of the journal Frontiers in Physiology These authors have contributed equally to this work. |
ISSN: | 1664-042X 1664-042X |
DOI: | 10.3389/fphys.2017.00449 |