Endothelium-Derived Relaxing Factor Reduces Platelet Adhesion to Bovine Endothelial Cells

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 85; no. 8; pp. 2800 - 2804
• Main Authors: Sneddon, John M., Vane, John R.
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.04.1988; National Acad Sciences
• Subjects: Adenine - metabolism; Adhesion; Animals; Aorta; Biological and medical sciences; Biological Products - physiology; Blood coagulation. Blood cells; Blood plasma; Blood Platelets - physiology; Bradykinin - pharmacology; Cattle; cell adhesion; Endothelial cells; endothelium; Endothelium, Vascular - cytology; Endothelium, Vascular - physiology; endothelium-derived relaxing factor; Fundamental and applied biological sciences. Psychology; Hemoglobins; Humans; In Vitro Techniques; Molecular and cellular biology; Nitric Oxide - pharmacology; Oxides; Platelet; Platelet Adhesiveness; Platelet aggregation; Platelets; Radioactive decay; Superoxide Dismutase - pharmacology; Thrombin - pharmacology; Ungulates; Aggregation; Blood coagulation; Vertebrata; Endothelial cell; Regulation(control); Platelet; Mammalia; Fixation; Bovine; Artiodactyla; Ungulata; Endothelium
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.85.8.2800

The adhesion of thrombin-stimulated human blood platelets to either the endothelial surface of intact bovine aorta or cultured bovine aortic endothelial cells was studied to determine the role of endothelium-derived relaxing factor in the regulation of platelet adhesion. Endothelial cells and platelets were pretreated with indomethacin to prevent the formation of prostaglandins. The adhesion of thrombin-stimulated platelets to endothelial cells was reduced by superoxide dismutase and bradykinin. The inhibitory effect of both drugs was abolished by hemoglobin and was absent in strips of bovine aorta where the endothelial cells had been removed by scraping. It is suggested that the effects of bradykinin are mediated by the release of endothelium-derived relaxing factor, which is protected from destruction by superoxide dismutase, and that endothelium-derived relaxing factor contributes to the nonadhesive properties of the vascular endothelium.