The Effect of Diet on Midgut and Resulting Changes in Infectiousness of AcMNPV Baculovirus in the Cabbage Looper, Trichoplusia ni

Insecticide resistance has been reported in many important agricultural pests, and alternative management methods are required. Baculoviruses qualify as an effective, yet environmentally benign, biocontrol agent but their efficacy against generalist herbivores may be influenced by diet. However, few...

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Published inFrontiers in physiology Vol. 9; p. 1348
Main Authors Chen, Elizabeth, Kolosov, Dennis, O'Donnell, Michael J, Erlandson, Martin A, McNeil, Jeremy N, Donly, Cam
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 04.10.2018
Subjects
AcMNPV baculovirus
cabbage looper
chitin deacetylase
chitinase
midgut transcriptome
pathogen resistance
Physiology
chitinase
peritrophic matrix
chitin deacetylase
midgut transcriptome
AcMNPV baculovirus
midgut pH
pathogen resistance
cabbage looper
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Summary:Insecticide resistance has been reported in many important agricultural pests, and alternative management methods are required. Baculoviruses qualify as an effective, yet environmentally benign, biocontrol agent but their efficacy against generalist herbivores may be influenced by diet. However, few studies have investigated the tritrophic interactions of plant, pest, and pathogen from both a gene expression and physiological perspective. Here we use microscopy and transcriptomics to examine how diet affects the structure of peritrophic matrix (PM) in larvae and consequently their susceptibility to the baculovirus, AcMNPV. Larvae raised on potato leaves had lower transcript levels for chitinase and chitin deacetylase genes, and possessed a thicker and more multi-layered PM than those raised on cabbage or artificial diet, which could contribute to their significantly lower susceptibility to the baculovirus. The consequences of these changes underline the importance of considering dietary influences on pathogen susceptibility in pest management strategies.
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This article was submitted to Invertebrate Physiology, a section of the journal Frontiers in Physiology
Edited by: Patrizia Falabella, University of Basilicata, Italy
Reviewed by: Yuan-Xi Li, Nanjing Agricultural University, China; Dandan Wei, Southwest University, China
ISSN:1664-042X
1664-042X
DOI:10.3389/fphys.2018.01348