Encapsulation of primary salivary gland cells in enzymatically degradable poly(ethylene glycol) hydrogels promotes acinar cell characteristics

• Published in: Acta biomaterialia Vol. 50; pp. 437 - 449
• Main Authors: Shubin, Andrew D., Felong, Timothy J., Schutrum, Brittany E., Joe, Debria S.L., Ovitt, Catherine E., Benoit, Danielle S.W.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.03.2017; Elsevier BV
• Subjects: Acinar cells; Acinar Cells - cytology; Animals; Aquaporin 5; Cell proliferation; Cell Size; Cells, Cultured; Cells, Immobilized - cytology; Chloride transport; Collagen (type IV); Crosslinking; Cytology; Damage; Degradability; Degradation; Encapsulation; Epithelial Cells - cytology; Female; Gene expression; Glands; Head and neck; Hydrogel; Hydrogels; Hydrogels - chemistry; In vitro methods and tests; Keratin-5 - metabolism; Laminin; Markers; Matrix metalloproteinase; Matrix Metalloproteinases - metabolism; Membrane proteins; Metalloproteinase; Mice, Inbred C57BL; Peptides; Poly(ethylene glycol); Polyethylene glycol; Polyethylene Glycols - chemistry; Proteins; Radiation; Radiation therapy; Regeneration; Salivary gland; Salivary glands; Salivary Glands - cytology; Self-assembly; Spheroids, Cellular - cytology; Submandibular gland; Substructures; Tight junctions; Tight Junctions - metabolism; Viability; Acinar cells; Degradation; Salivary gland; Hydrogel; Poly(ethylene glycol)
• ISSN: 1742-7061; 1878-7568
• DOI: 10.1016/j.actbio.2016.12.049

[Display omitted] Radiation therapy for head and neck cancers leads to permanent xerostomia due to the loss of secretory acinar cells in the salivary glands. Regenerative treatments utilizing primary submandibular gland (SMG) cells show modest improvements in salivary secretory function, but there is limited evidence of salivary gland regeneration. We have recently shown that poly(ethylene glycol) (PEG) hydrogels can support the survival and proliferation of SMG cells as multicellular spheres in vitro. To further develop this approach for cell-based salivary gland regeneration, we have investigated how different modes of PEG hydrogel degradation affect the proliferation, cell-specific gene expression, and epithelial morphology within encapsulated salivary gland spheres. Comparison of non-degradable, hydrolytically-degradable, matrix metalloproteinase (MMP)-degradable, and mixed mode-degradable hydrogels showed that hydrogel degradation by any mechanism is required for significant proliferation of encapsulated cells. The expression of acinar phenotypic markers Aqp5 and Nkcc1 was increased in hydrogels that are MMP-degradable compared with other hydrogel compositions. However, expression of secretory acinar proteins Mist1 and Pip was not maintained to the same extent as phenotypic markers, suggesting changes in cell function upon encapsulation. Nevertheless, MMP- and mixed mode-degradability promoted organization of polarized cell types forming tight junctions and expression of the basement membrane proteins laminin and collagen IV within encapsulated SMG spheres. This work demonstrates that cellularly remodeled hydrogels can promote proliferation and gland-like organization by encapsulated salivary gland cells as well as maintenance of acinar cell characteristics required for regenerative approaches. Investigation is required to identify approaches to further enhance acinar secretory properties. Regenerative strategies to replace damaged salivary glands require the function and organization of acinar cells. Hydrogel-based approaches have shown promise to control cell function and phenotype. However, little is known about how specific parameters, such as the mechanism of hydrogel degradation (e.g., hydrolytic or enzymatic), influence the viability, proliferation, organization, and phenotype of salivary gland cells. In this work, it is shown that hydrogel-encapsulated primary salivary gland cell proliferation is dependent upon hydrogel degradation. Hydrogels crosslinked with enzymatically degradable peptides promoted the expression of critical acinar cell markers, which are typically downregulated in primary cultures. Furthermore, salivary gland cells encapsulated in enzymatically- but not hydrolytically-degradable hydrogels displayed highly organized and polarized salivary gland cell markers, which mimics characteristics found in native gland tissue. In sum, results indicate that salivary gland cells respond to cellularly remodeled hydrogels, resulting in self-assembly and organization akin to acini substructures of the salivary gland.