C-Type Lectin Receptor (CLR)-Fc Fusion Proteins As Tools to Screen for Novel CLR/Bacteria Interactions: An Exemplary Study on Preselected Campylobacter jejuni Isolates

• Published in: Frontiers in immunology Vol. 9; p. 213
• Main Authors: Mayer, Sabine, Moeller, Rebecca, Monteiro, João T, Ellrott, Kerstin, Josenhans, Christine, Lepenies, Bernd
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 13.02.2018
• Subjects: C-type lectin receptors; Campylobacter jejuni; Campylobacter jejuni - immunology; confocal microscopy; ELISA assay; Enzyme-Linked Immunosorbent Assay - methods; flow cytometry; Flow Cytometry - methods; Host-Parasite Interactions - immunology; Humans; Immunology; innate immunity; Lectins, C-Type - genetics; Lectins, C-Type - immunology; Lectins, C-Type - isolation & purification; Microscopy, Confocal; Receptors, Fc - genetics; Receptors, Fc - immunology; Receptors, Fc - isolation & purification; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - immunology; Recombinant Fusion Proteins - isolation & purification; C-type lectin receptors; innate immunity; confocal microscopy; screening tools; Dectin-1 receptor; flow cytometry; ELISA assay; Campylobacter jejuni
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2018.00213

C-type lectin receptors (CLRs) are carbohydrate-binding receptors that recognize their ligands often in a Ca -dependent manner. Upon ligand binding, myeloid CLRs in innate immunity trigger or inhibit a variety of signaling pathways, thus initiating or modulating effector functions such as cytokine production, phagocytosis, and antigen presentation. CLRs bind to various pathogens, including viruses, fungi, parasites, and bacteria. The bacterium ( ) is a very frequent Gram-negative zoonotic pathogen of humans, causing severe intestinal symptoms. Interestingly, expresses several glycosylated surface structures, for example, the capsular polysaccharide (CPS), lipooligosaccharide (LOS), and envelope proteins. This "Methods" paper describes applications of CLR-Fc fusion proteins to screen for yet unknown CLR/bacteria interactions using as an example. ELISA-based detection of CLR/bacteria interactions allows a first prescreening that is further confirmed by flow cytometry-based binding analysis and visualized using confocal microscopy. By applying these methods, we identified Dectin-1 as a novel CLR recognizing two selected isolates with different LOS and CPS genotypes. In conclusion, the here-described applications of CLR-Fc fusion proteins represent useful methods to screen for and identify novel CLR/bacteria interactions.