Analysis of Bacterial Community Characteristics, Abundance of Antibiotics and Antibiotic Resistance Genes Along a Pollution Gradient of Ba River in Xi'an, China

• Published in: Frontiers in microbiology Vol. 9; p. 3191
• Main Authors: Guan, Yongjing, Jia, Jia, Wu, Lang, Xue, Xue, Zhang, Guo, Wang, Zaizhao
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 21.12.2018
• Subjects: antibiotic resistance genes; antibiotics; bacterial community; Microbiology; resident domestic sewage; urban river; wastewater treatment plant; urban river; bacterial community; antibiotic resistance genes; wastewater treatment plant; antibiotics; resident domestic sewage
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2018.03191

The microbial communities in freshwater have raised concerns about the ecosystem and human health. Many ecological environmental problems have been found in urban river because of the unreasonable use and long-term wastewater discharge. In this study, we explored the bacterial community composition, abundance of 14 antibiotics and 21 antibiotic resistance genes (ARGs), and water environment features in seven water samples and seven sediment samples from Ba River in Xi'an, China. Results showed and were the dominant phyla in all samples, and sediment samples had a higher bacterial diversity and richness than it in water. Bacterial communities of site 5 and 6 were clustered in discrepant patterns compared to those at remaining sites from other samples. It might be influenced by nutrients, heavy metals and antibiotics. Antibiotics concentrations ranged from 1.26 to 1.61 × 10 ng L in water samples and 1.55 to 4.05 × 10 μg kg in sediment samples. Sulfamerazine (SM1) and erythromycin (ERY) were the chief antibiotics in water samples, while the level of oxytetracycline (OTC) and cefazolin (CFZ) were higher in sediment samples. Canonical correspondence analysis showed that trimethoprim (TMP) was significantly related to in W6, and that SM1 and OTC had positive correlation with in W5. The and had higher pollution abundance ranging from 10 to 10 copies/16S rRNA gene copies in all samples. Significant correlations were observed between ARGs and matching antibiotics, suggesting that antibiotics can pose the selective pressure on ARGs in this river. In summary, these finding might provide some new data to the limited information available on the bacterial community characteristics, abundance of antibiotics and ARGs in urban river of China.