Binding site of restriction-modification system controller protein in Mollicutes

• Published in: BMC microbiology Vol. 17; no. 1; p. 26
• Main Authors: Fisunov, Gleb Y, Evsyutina, Daria V, Manuvera, Valentin A, Govorun, Vadim M
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 31.01.2017; BioMed Central
• Subjects: Bacteria; Bacterial proteins; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Binding sites; Binding Sites - physiology; Chromosome Mapping; Cloning; DNA methylation; DNA Restriction-Modification Enzymes - genetics; DNA Restriction-Modification Enzymes - metabolism; Endopeptidase Clp - genetics; Gene expression; Gene Expression Regulation, Bacterial - genetics; Gene Expression Regulation, Bacterial - physiology; Genes, Bacterial; Genomes; Kinases; Mollicutes; Mycoplasma gallisepticum - genetics; Mycoplasma gallisepticum - metabolism; Observations; Operon - physiology; Physiological aspects; Promoter Regions, Genetic; Protein Binding; Protein Processing, Post-Translational; Proteins; RNA, Transfer - metabolism; Tenericutes - genetics; Tenericutes - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism; Transcription, Genetic - physiology; Mollicutes; Regulation; Restriction-modification; Mycoplasma; Transcription factor
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-017-0935-4

Bacteria of the class Mollicutes underwent extreme reduction of genomes and gene expression control systems. Only a few regulators are known to date. In this work, we describe a novel group of transcriptional regulators that are distributed within different Mollicutes and control the expression of restriction-modification systems (RM-systems). We performed cross-species search of putative regulators of RM-systems (C-proteins) and respective binding sites in Mollicutes. We identified a set of novel putative C-protein binding motifs distributed within Mollicutes. We studied the most frequent motif and respective C-protein on the model of Mycoplasma gallisepticum S6. We confirmed our prediction and identified key nucleotides important for C-protein binding. Further we identified novel target promoters of C-protein in M. gallisepticum. We found that C-protein of M. gallisepticum binds predicted conserved direct repeats of the (GTGTTAN ) motif. Apart from its own operon promoter, HsdC can bind to the promoters of the clpB chaperone gene and a tRNA cluster.