Actinomycetes isolated from medicinal plant rhizosphere soils: diversity and screening of antifungal compounds, indole-3-acetic acid and siderophore production

• Published in: World journal of microbiology & biotechnology Vol. 25; no. 4; pp. 649 - 655
• Main Authors: Khamna, Sutthinan, Yokota, Akira, Lumyong, Saisamorn
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.04.2009; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Acetic acid; Acids; Actinomadura; agar; Alternaria brassicicola; antifungal agents; Applied Microbiology; Athelia rolfsii; Biochemistry; Biodiversity; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Curcuma; Curcuma mangga; Environmental Engineering/Biotechnology; Environmental impact; Fundamental and applied biological sciences. Psychology; Fungi; Fusarium oxysporum; Gram-positive bacteria; Herbal medicine; indole acetic acid; Life Sciences; Medicinal plants; Metabolites; Microbiology; Microbispora; Micromonospora; Microorganisms; Morphology; Nocardia; Original Paper; Pathogens; Penicillium digitatum; Plant diseases; Plant growth; plant pathogenic fungi; Rhizosphere; Sclerotium rolfsii; screening; siderophores; soil; Soils; Streptomyces; Studies; Yeast; Siderophores; Actinomycetes; Indole-3-acetic acid; Antagonistic; Biocontrol; Indole; Biological control; Diversity; Medicinal plant; Screening; Rhizosphere; Soils; Antifungal agent; Siderophore; Production; Bacteria; Antagonist; Acetic acid
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-008-9933-x

A total of 445 actinomycete isolates were obtained from 16 medicinal plant rhizosphere soils. Morphological and chemotaxonomic studies indicated that 89% of the isolates belonged to the genus Streptomyces, 11% were non-Streptomycetes: Actinomadura sp., Microbispora sp., Micromonospora sp., Nocardia sp, Nonomurea sp. and three isolates were unclassified. The highest number and diversity of actinomycetes were isolated from Curcuma mangga rhizosphere soil. Twenty-three Streptomyces isolates showed activity against at least one of the five phytopathogenic fungi: Alternaria brassicicola, Collectotrichum gloeosporioides, Fusarium oxysporum, Penicillium digitatum and Sclerotium rolfsii. Thirty-six actinomycete isolates showed abilities to produce indole-3-acetic acid (IAA) and 75 isolates produced siderophores on chrome azurol S (CAS) agar. Streptomyces CMU-PA101 and Streptomyces CMU-SK126 had high ability to produced antifungal compounds, IAA and siderophores.