Euphane triterpenoids of Cassipourea lanceolata from the Madagascar rainforest

• Published in: Phytochemistry (Oxford) Vol. 71; no. 5; pp. 669 - 674
• Main Authors: Hou, Yanpeng, Cao, Shugeng, Brodie, Peggy J., Miller, James S., Birkinshaw, Chris, Andrianjafy, Mamisoa N., Andriantsiferana, Rabodo, Rasamison, Vincent E., TenDyke, Karen, Shen, Yongchun, Suh, Edward M., Kingston, David G.I.
• Format: Journal Article
• Language: English
• Published: OXFORD Elsevier Ltd 01.04.2010; Elsevier
• Subjects: anticarcinogenic activity; Antineoplastic Agents, Phytogenic - chemistry; Antineoplastic Agents, Phytogenic - isolation & purification; Antineoplastic Agents, Phytogenic - pharmacology; Antiproliferative activity; Biochemistry & Molecular Biology; Biological and medical sciences; Cassipourea lanceolata; Cell Line, Tumor; cell proliferation; Chemical constitution; chemical structure; cytotoxicity; esters; euphadiene; euphane triterpenes; Euphanes; Female; forest trees; Fruit; Fundamental and applied biological sciences. Psychology; Humans; Inhibitory Concentration 50; Life Sciences & Biomedicine; Madagascar; Molecular Structure; NMR; Ovarian Neoplasms - drug therapy; phytochemicals; Phytotherapy; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves; Plant physiology and development; Plant Sciences; rain forests; Rhizophoraceae; Rhizophoraceae - chemistry; Science & Technology; spectral analysis; stereochemistry; Triterpenes - chemistry; Triterpenes - isolation & purification; Triterpenes - pharmacology; Triterpenoids; Madagascar; Malagasy Republic; Africa; NMR; Euphanes; Cassipourea lanceolata; Antiproliferative activity; Triterpenoids; Rhizophoraceae; GUIANENSIS; GERRARDII; ALKALOIDS; Antineoplastic agent; Terpenoid; Chemical structure; Dicotyledones; Angiospermae; Plant origin; Triterpene; Spermatophyta
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/j.phytochem.2009.12.009

A Madagascar collection of the leaves and fruit of Cassipourea lanceolata Tul. led to the isolation of three euphane triterpenoids 1– 3. The three compounds showed weak antiproliferative activities against the A2780 human ovarian cancer cell line, with IC 50 values of 25, 25 and 32 μM, respectively. Fractionation of an ethanol extract of a Madagascar collection of the leaves and fruit of Cassipourea lanceolata Tul. led to the isolation of three euphane triterpenoids 1– 3. The 1H and 13C NMR spectra of all compounds were fully assigned using a combination of 2D NMR experiments, including COSY, TOCSY, HSQC (HMQC), HMBC and ROESY sequences. The three compounds showed weak antiproliferative activities against the A2780 human ovarian cancer cell line, with IC 50 values of 25, 25 and 32 μM, respectively.