Proteins Involved in the Induction of Procoagulant Activity and Autoimmune Response in Patients With Primary Antiphospholipid Syndrome

• Published in: Clinical and applied thrombosis/hemostasis Vol. 26; p. 1076029620905338
• Main Authors: Araújo, Débora Medeiros, Rodrigues, Carlos Ewerton Maia, Gonçalves, Nidyedja Goyanna Gomes, Rabelo-Júnior, Carlos Nobre, Lobo, Marina Duarte Pinto, Moreira, Renato de Azevedo, Monteiro-Moreira, Ana Cristina de Oliveira
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 2020; SAGE PUBLICATIONS, INC; SAGE Publishing
• Subjects: Adult; Antiphospholipid Syndrome - blood; Autoimmune diseases; Biomarkers for Evaluating the Risk and Prognosis of Vascular Diseases; Female; Humans; Male; Mass Spectrometry - methods; Proteins; Proteins - metabolism; Proteomics - methods; antiphospholipid syndrome; mass spectrometry; protein profile
• ISSN: 1076-0296; 1938-2723
• DOI: 10.1177/1076029620905338

The aim of this study was to determine the plasma protein profile of patients with primary antiphospholipid syndrome (PAPS) compared to healthy controls and identify proteins that might be used in the evaluation, diagnosis, and prognosis of this condition. The sample consisted of 14 patients with PAPS and 17 sex- and age-matched controls. Plasma samples were submitted to proteomic analysis (albumin and immunoglobulin G depletion, concentration, digestion, and label-free data-independent mass spectrometry). The software ExpressionE was used to quantify intergroup differences in protein expression. The analysis yielded 65 plasma proteins of which 11 were differentially expressed (9 upregulated and 2 downregulated) in relation to controls. Four of these are known to play a role in pathophysiological mechanisms of thrombosis: fibrinogen α chain, fibrinogen α chain, apolipoprotein C-III, and α-1-glycoprotein-1. Our analysis revealed autoimmune response and the presence of proteins believed to be functionally involved in the induction of procoagulant activity in patients with PAPS. Further studies are necessary to confirm our findings and may eventually lead to the development of significantly more accurate diagnostic tools.