Comparison of Multiple-Locus Variable-Number Tandem-Repeat Analysis with Other PCR-Based Methods for Typing Brucella suis Isolates

• Published in: Journal of Clinical Microbiology Vol. 45; no. 12; pp. 4070 - 4072
• Main Authors: García-Yoldi, David, Le Fleche, Philippe, De Miguel, María J, Muñoz, Pilar M, Blasco, José M, Cvetnic, Zeljko, Marín, Clara M, Vergnaud, Gilles, López-Goñi, Ignacio
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.12.2007; American Society for Microbiology (ASM)
• Subjects: Animals; Animals, Wild - microbiology; Bacterial Typing Techniques - methods; Bacteriology; Biochemistry, Molecular Biology; Biological and medical sciences; Brucella suis; Brucella suis - classification; Brucella suis - genetics; Brucellosis - epidemiology; Brucellosis - microbiology; Cellular Biology; Cluster Analysis; Disease Outbreaks; DNA Fingerprinting - methods; DNA, Bacterial - genetics; Fundamental and applied biological sciences. Psychology; Life Sciences; Microbiology; Minisatellite Repeats; Miscellaneous; Polymerase Chain Reaction - methods; Polymorphism, Restriction Fragment Length; Tandem Repeat Sequences - genetics; Polymerase chain reaction; Typing; Brucellaceae; Minisatellite DNA; Bacteria; Isolate; Method; Brucella suis
• ISSN: 0095-1137; 1098-660X; 1098-5530
• DOI: 10.1128/JCM.01096-07

Multiple-locus variable-number tandem-repeat analysis (MLVA), multiplex PCR, and PCR-restriction fragment length polymorphism analysis were compared for typing Brucella suis isolates. A perfect concordance was obtained among these molecular assays. However, MLVA was the only method to demonstrate brucellosis outbreaks and to confirm that wildlife is a reservoir for zoonotic brucellosis.