Molecular and Biological Characterization of Ralstonia Phage RsoM1USA, a New Species of P2virus , Isolated in the United States

The first -infecting bacteriophage from soil of the United States, designated RsoM1USA, was isolated from a tomato field in Florida. Electron microscopy revealed that phage RsoM1USA is member of the genus in the family with an icosahedral head of about 66 nm in diameter, a contractile tail of about...

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Published inFrontiers in microbiology Vol. 10; p. 267
Main Authors Addy, Hardian Susilo, Ahmad, Abdelmonim Ali, Huang, Qi
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 19.02.2019
Subjects
characterization
isolation
Microbiology
Myoviridae
P2virus
Ralstonia phage
Ralstonia solanacearum
Ralstonia phage
isolation
Ralstonia solanacearum
characterization
Myoviridae
P2virus
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Summary:The first -infecting bacteriophage from soil of the United States, designated RsoM1USA, was isolated from a tomato field in Florida. Electron microscopy revealed that phage RsoM1USA is member of the genus in the family with an icosahedral head of about 66 nm in diameter, a contractile tail of about 152 nm in length, and a long "neck." Phage RsoM1USA infected 12 of the 30 tested species complex strains collected worldwide in each of the three species: , , and . The phage completed its infection cycle 180 min post infection with a burst size of about 56 particles per cell. Phage RsoM1USA has a genome of 39,309 nucleotides containing 58 open reading frames (ORFs) and is closely related to Ralstonia phage RSA1 of the species . The genomic organization of phage RsoM1USA is also similar to that of phage RSA1, but their integrases share no sequence homology. In addition, we determined that the integration of phage RsoM1USA into its susceptible strain K60 is mediated by the 3' 45-base portion of the threonine tRNA (TGT), not arginine tRNA (CCG) as reported for phage RSA1, confirming that the two phages use different mechanism for integration. Our proteomic analysis of the purified virions supported the annotation of the main structural proteins. Infection of a susceptible strain RUN302 by phage RsoM1USA resulted in significantly reduced growth of the infected bacterium , but not virulence in tomato plants, as compared to its uninfected RUN302 strain. Due to its differences from phage RSA1, phage RsoM1USA should be considered the type member of a new species with a proposed species name of
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Edited by: Ramy Karam Aziz, Cairo University, Egypt
This article was submitted to Virology, a section of the journal Frontiers in Microbiology
Reviewed by: Jessica Sacher, University of Alberta, Canada; Hany Anany, Agriculture and Agri-Food Canada (AAFC), Canada
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2019.00267