Lipoteichoic Acid of Probiotic Lactobacillus plantarum Attenuates Poly I:C-Induced IL-8 Production in Porcine Intestinal Epithelial Cells

Probiotics in livestock feed supplements are considered a replacement for antibiotics that enhance gastrointestinal immunity. Although bacterial cell wall components have been proposed to be associated with probiotic function, little evidence demonstrates that they are responsible for probiotic func...

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Published inFrontiers in microbiology Vol. 8; p. 1827
Main Authors Kim, Kyoung Whun, Kang, Seok-Seong, Woo, Sun-Je, Park, Ok-Jin, Ahn, Ki Bum, Song, Ki-Duk, Lee, Hak-Kyo, Yun, Cheol-Heui, Han, Seung Hyun
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 21.09.2017
Subjects
inflammation
intestinal epithelial cells
lipoteichoic acid
Microbiology
probiotics
viral infection
lipoteichoic acid
intestinal epithelial cells
viral infection
inflammation
probiotics
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Summary:Probiotics in livestock feed supplements are considered a replacement for antibiotics that enhance gastrointestinal immunity. Although bacterial cell wall components have been proposed to be associated with probiotic function, little evidence demonstrates that they are responsible for probiotic functions in livestock. The present study demonstrated that lipoteichoic acid (LTA) of (Lp.LTA) confers anti-inflammatory responses in porcine intestinal epithelial cell line, IPEC-J2. A synthetic analog of viral double-stranded RNA, poly I:C, dose-dependently induced IL-8 production at the mRNA and protein levels in IPEC-J2 cells. Lp.LTA, but not lipoprotein or peptidoglycan from , exclusively suppressed poly I:C-induced IL-8 production. Compared with LTAs from other probiotic strains including , , and GG, Lp.LTA had higher potential to suppress poly I:C-induced IL-8 production. Dealanylated or deacylated Lp.LTA did not suppress poly I:C-induced IL-8 production, suggesting that D-alanine and lipid moieties in the Lp.LTA structure were responsible for the inhibition. Furthermore, Lp.LTA attenuated the phosphorylation of ERK and p38 kinase as well as the activation of NF-κB, resulting in decreased IL-8 production. Taken together, these results suggest that Lp.LTA acts as an effector molecule to inhibit viral pathogen-induced inflammatory responses in porcine intestinal epithelial cells.
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Edited by: Aldo Corsetti, Università di Teramo, Italy
This article was submitted to Food Microbiology, a section of the journal Frontiers in Microbiology
These authors have contributed equally to this work.
Reviewed by: Maria Guadalupe Vizoso Pinto, National University of Tucumán, Argentina; Cristian Botta, University of Turin, Italy
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2017.01827