Next-Generation Sequencing of Antibody Display Repertoires

selection technology has transformed the development of therapeutic monoclonal antibodies. Using methods such as phage, ribosome, and yeast display, high affinity binders can be selected from diverse repertoires. Here, we review strategies for the next-generation sequencing (NGS) of phage- and other...

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Bibliographic Details
Published inFrontiers in immunology Vol. 9; p. 118
Main Authors Rouet, Romain, Jackson, Katherine J L, Langley, David B, Christ, Daniel
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 02.02.2018
Subjects
Antibodies
Antibody Affinity
antibody display technology
antibody libraries
antibody therapeutics
Bacteriophages
Epitope Mapping
High-Throughput Nucleotide Sequencing
Humans
Immunology
in vitro selection
next-generation sequencing
phage display
antibody therapeutics
antibody libraries
in vitro selection
next-generation sequencing
phage display
antibody display technology
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Summary:selection technology has transformed the development of therapeutic monoclonal antibodies. Using methods such as phage, ribosome, and yeast display, high affinity binders can be selected from diverse repertoires. Here, we review strategies for the next-generation sequencing (NGS) of phage- and other antibody-display libraries, as well as NGS platforms and analysis tools. Moreover, we discuss recent examples relating to the use of NGS to assess library diversity, clonal enrichment, and affinity maturation.
Bibliography:ObjectType-Article-2
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Edited by: Prabakaran Ponraj, Intrexon, United States
Specialty section: This article was submitted to B Cell Biology, a section of the journal Frontiers in Immunology
Reviewed by: Yang Feng, National Cancer Institute at Frederick, United States; Masaki Hikida, Kyoto University, Japan
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2018.00118