Purification of innate immunostimulant from green tea using a silkworm muscle contraction assay

A polysaccharide was purified from a hot water extract of green tea leaves by measuring the immunostimulatory activity in silkworm larvae. Nuclear magnetic resonance and chemical analysis of acid hydrolysates revealed that the purified substance possessed a backbone containing polygalacturonic acids...

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Published inDrug Discoveries & Therapeutics Vol. 5; no. 1; pp. 18 - 25
Main Authors Dhital, Saphala, Hamamoto, Hiroshi, Urai, Makoto, Ishii, Kenichi, Sekimizu, Kazuhisa
Format Journal Article
LanguageEnglish
Published Japan International Research and Cooperation Association for Bio & Socio-Sciences Advancement 01.01.2011
Subjects
green tea
IL-6
innate immunity
insect
macrophages
mice
pectin
Polysaccharides
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Summary:A polysaccharide was purified from a hot water extract of green tea leaves by measuring the immunostimulatory activity in silkworm larvae. Nuclear magnetic resonance and chemical analysis of acid hydrolysates revealed that the purified substance possessed a backbone containing polygalacturonic acids with methyl ester residues. Treatment with β-glucanase attenuated the muscle contraction activity of the purified sample, suggesting that the β-glucan structure, probably as a branched form, was required for its activity. The purified fraction stimulated the production of interleukin-6 by mouse peritoneal macrophages. These results suggest that measuring immunostimulation in silkworm larvae is useful for evaluating innate immunostimulants from various sources.
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ISSN:1881-7831
1881-784X
DOI:10.5582/ddt.v5.1.18