The Comparison of Two Different Originated Peptide in Facilitating Recovery of Exercise-induced Muscular Fatigue

• Published in: FOOD SCIENCE AND TECHNOLOGY RESEARCH Vol. 30; no. 6; pp. 669 - 678
• Main Authors: Liu, Dansong, Wang, Bei, Han, Qi, Yu, Aiqi, Gao, Ziyu, Shao, Jing, Li, Tieying, Li, Shuang, Sui, Xuemei, Duan, Shenglin, Wang, Qirong
• Format: Journal Article
• Language: English
• Published: Tsukuba Japanese Society for Food Science and Technology 01.01.2024; The Japanese Society for Food Science and Technology; Japan Science and Technology Agency
• Subjects: Calpain; exercise-induced muscle damage; Insulin-like growth factor I; Insulin-like growth factors; marine collagen peptide; Muscles; Muscular fatigue; Musculoskeletal system; Peptides; Quadriceps muscle; Recovery; Skeletal muscle; soy peptide; Testosterone
• ISSN: 1344-6606; 1881-3984
• DOI: 10.3136/fstr.FSTR-D-23-00168

The purpose of this study was to compare the effects of two different originated peptide, namely, marine collagen peptide (MCP) and soy peptide (SP) on Calpain-1 (CAPN-1) and Calpain-2 (CAPN-2) and skeletal muscle micro-injury. Method: One hundred male SD rats were divided into four groups: control group (Group C, n = 10), exercise + MCP group (Group M, n = 30), exercise + SP group (Group S, n = 30), and exercise group (Group E, n = 30). After six weeks of training, blood and skeletal muscle were taken immediately, 24 h, and 48 h after exercise. IGF-1, CK, testosterone (T), and skeletal muscle expression of CAPN-1 and CAPN-2 were tested. Results: IGF-1 of Group S was greater immediately after exercise compared to Group E (p < 0.05), and T recovery was faster in Group M than E (p < 0.05). CK was upregulated in Groups E and M immediately after exercise, while Group S had greater CK than Groups E and C (p < 0.05). Group M had higher CAPN-1 expression in quadriceps than Groups E and S (p < 0.05) immediately after exercise. CAPN-2 expression in gastrocnemius was upregulated immediately after exercise. T was negatively correlated with CAPN-2 expression in gastrocnemius (r = −0.715, p < 0.05) immediately after exercise, and it was negatively correlated with CAPN-1 expression in quadriceps (r = −0.905, p < 0.05) 24 h after exercise. Conclusion: Both peptides demonstrated limited effect on post-exercise CK, CAPN-1, and CAPN-2. Compared with SP, MCP had a more significant effect on recovery of T after exercise. And SP was more effective than MCP in downregulating exercise-induced upregulation of CAPN-1 and CAPN-2 expression observed 48 h after exercise in gastrocnemius.