Biodiesel synthesis and conformation of lipase from Burkholderia cepacia in room temperature ionic liquids and organic solvents

• Published in: Bioresource technology Vol. 102; no. 22; pp. 10414 - 10418
• Main Authors: Liu, Yun, Chen, Dawei, Yan, Yunjun, Peng, Cheng, Xu, Li
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.11.2011; Elsevier
• Subjects: Anions; Biocatalysis; Biodiesel; Biofuel production; Biofuels - analysis; Biological and medical sciences; Biotechnology; Burkholderia; Burkholderia cepacia; Burkholderia cepacia - enzymology; Burkholderia cepacia lipase (BCL); Cations; Conversion; Energy; Esterification; Fourier transform-infrared spectrometry (FT-IR); Fundamental and applied biological sciences. Psychology; Industrial applications and implications. Economical aspects; Ionic liquids; Ionic Liquids - chemistry; Lipase; Lipase - chemistry; Lipase - metabolism; Organic Chemicals - chemistry; Protein Structure, Secondary; Room temperature ionic liquids (RTILs); Secondary structure; Spectroscopy, Fourier Transform Infrared; Synthesis; Temperature; Ultrasonics; Room temperature ionic liquids (RTILs); Burkholderia cepacia lipase (BCL); Secondary structure; Biodiesel; Fourier transform-infrared spectrometry (FT-IR); Enzyme; FT-IR; Ionic liquid; Organic solvent; Fourier transform-infrared spectrometry; Burkholderia cepacia; Bacteria; Fourier-transformed infrared spectrometry; Room temperature; Lipolytic enzyme
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2011.08.056

► Biodiesel production by the lipase from Burkholderia cepacia (BCL) was investigated in 19 different ionic liquids (ILs). ► Influence of 5 different organic solvents on biodiesel synthesis by BCL was also evaluated as references. ► Relationship between conformation and activity of BCL by FT-IR was elucidated in the above-mentioned ILs and organic solvents. Biodiesel synthesis and conformation of Burkholderia cepacia lipase (BCL) were studied in 19 different room temperature ionic liquids (RTLLs) with a range of cation and anion structures. Overall, anion selection had a greater influence on biodiesel conversion than cation choice. RTILs containing Tf2N−and PF6- anions were suitable reaction media, while RTIL of [OmPy][BF4] was the best reaction medium with a biodiesel yield of 82.2±1.2%. RTILs with strong water miscible properties showed very low biodiesel yields. Conformational analysis by FT-IR revealed that higher biodiesel conversion in RTILs was correlated with a low tendency in α-helix content of BCL. An ultrasound-assisted biocatalysis process in RTILs was used to improve mass transfer rate, leading to 83% reduction of the reaction time for biodiesel production.