Method detection limits of PCR and immunofluorescence assay for Cryptosporidium parvum in soil
We determined and compared the method detection limits (MDL alpha) of a PCR and an immunofluorescence assay (IFA) for detection of Cryptosporidium parvum oocysts in soils. Based on the MDL alpha and the quantitative nature and stability of the IFA, PCR analysis is not a useful screening step for soi...
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Published in | Applied and Environmental Microbiology Vol. 64; no. 6; pp. 2281 - 2283 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Washington, DC
American Society for Microbiology
01.06.1998
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Subjects | |
Online Access | Get full text |
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Summary: | We determined and compared the method detection limits (MDL alpha) of a PCR and an immunofluorescence assay (IFA) for detection of Cryptosporidium parvum oocysts in soils. Based on the MDL alpha and the quantitative nature and stability of the IFA, PCR analysis is not a useful screening step for soil studies of oocyst transport |
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Bibliography: | 1997093100 L72 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 Corresponding author. Mailing address: Department of Environmental and Resource Sciences, University of Nevada, Reno, NV 89557-0013. Phone: (702) 784-6763. Fax: (702) 784-4789. E-mail: mwalker@equinox.unr.edu. |
ISSN: | 0099-2240 1098-5336 |
DOI: | 10.1128/aem.64.6.2281-2283.1998 |