The Cis-Acting RNA Trafficking Signal from Myelin Basic Protein mRNA and Its Cognate Trans-Acting Ligand hnRNP A2 Enhance Cap-Dependent Translation

The 21 nucleotide RNA trafficking signal (RTS), originally identified in myelin basic protein mRNA, but also found in a variety of other localized RNAs, is necessary and sufficient for transport of RNA along microtubules in oligodendrocytes. The RTS binds specifically to the RNA binding protein, hnR...

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Published inThe Journal of cell biology Vol. 147; no. 2; pp. 247 - 256
Main Authors Kwon, Sunjong, Barbarese, Elisa, Carson, John H.
Format Journal Article
LanguageEnglish
Published United States Rockefeller University Press 18.10.1999
The Rockefeller University Press
Subjects
Animals
Cell nucleus
Cells
Cellular biology
CHO Cells
Cricetinae
Efficiency metrics
Fluorescence
Heterogeneous nuclear ribonucleoproteins
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
hnRNA
Messenger RNA
Myelin Basic Protein - genetics
Neurons
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Oligodendroglia
Original
Protein Biosynthesis
Proteins
Ribonucleic acid
Ribonucleoproteins - genetics
Ribonucleoproteins - metabolism
RNA
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
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Summary:The 21 nucleotide RNA trafficking signal (RTS), originally identified in myelin basic protein mRNA, but also found in a variety of other localized RNAs, is necessary and sufficient for transport of RNA along microtubules in oligodendrocytes. The RTS binds specifically to the RNA binding protein, hnRNP A2. Together, the RTS and hnRNP A2 comprise cis/trans determinants for several steps in the RNA trafficking pathway. Here we show that insertion of the RTS into green fluorescent protein (GFP) RNA enhances translation without affecting stability of microinjected RNA. In dicistronic RNA, the RTS enhances cap-dependent translation without affecting internal ribosome entry site (IRES)-dependent translation. The translation enhancer function of the RTS is position, copy number, and cell type independent, hnRNP A2 dependent, and saturable with increasing amounts of injected RNA. This represents one of the first specific translation enhancer elements identified in a mammalian system.
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ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.147.2.247