Genetic variation in phosphodiesterase (PDE) 7B in chronic lymphocytic leukemia: overview of genetic variants of cyclic nucleotide PDEs in human disease

• Published in: Journal of human genetics Vol. 56; no. 9; pp. 676 - 681
• Main Authors: Peiró, Ana M, Tang, Chih-Min, Murray, Fiona, Zhang, Lingzhi, Brown, Loren M, Chou, Daisy, Rassenti, Laura, Kipps, Thomas A, Insel, Paul A
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.09.2011; Nature Publishing Group
• Subjects: 5' Untranslated Regions; 5' Untranslated Regions - genetics; 631/208/726/649; 692/699/67/1990/283/1895; Adult; Aged; Agent Orange; Biomedical and Life Sciences; Biomedicine; Chronic lymphocytic leukemia; Cyclic AMP; Cyclic Nucleotide Phosphodiesterases, Type 7 - genetics; Cyclic Nucleotide Phosphodiesterases, Type 7 - metabolism; Female; Gene Expression; Gene Frequency; Gene Function; Gene Therapy; Genetic diversity; Human Genetics; Humans; Leukemia; Leukemia, Lymphocytic, Chronic, B-Cell - genetics; Leukemia, Lymphocytic, Chronic, B-Cell - metabolism; Lymphatic leukemia; Male; Middle Aged; Molecular Medicine; mRNA; original-article; Phosphodiesterase; Polymorphism, Single Nucleotide - genetics; Promoter Regions, Genetic - genetics; RNA, Messenger - genetics; RNA, Messenger - metabolism; Single-nucleotide polymorphism; PDE7B single-nucleotide polymorphisms; chronic lymphocytic leukemia; cAMP; cyclic nucleotide phosphodiesterases
• ISSN: 1434-5161; 1435-232X; 1435-232X
• DOI: 10.1038/jhg.2011.80

Expression of cyclic adenosine monophosphate-specific phosphodiesterase 7B (PDE7B) mRNA is increased in patients with chronic lymphocytic leukemia (CLL), thus suggesting that variation may occur in the PDE7B gene in CLL. As genetic variation in other PDE family members has been shown to associate with numerous clinical disorders (reviewed in this manuscript), we sought to identify single-nucleotide polymorphisms (SNPs) in the PDE7B gene promoter and coding region of 93 control subjects and 154 CLL patients. We found that the PDE7B gene has a 5′ non-coding region SNP −347C>T that occurs with similar frequency in CLL patients (1.9%) and controls (2.7%). Tested in vitro , −347C>T has less promoter activity than a wild-type construct. The low frequency of this 5′ untranslated region variant indicates that it does not explain the higher PDE7B expression in patients with CLL but it has the potential to influence other settings that involve a role for PDE7B.